| Objective: 1 To investigate the coagulation factor Ⅻ activity and anticardiolipin antibody in patients suffering from unexplained recurrent spontaneous abortion in order to determine if factor Ⅻ activity deficiency to be a predictor of unexplained recurrent spontaneous abortion and is there a relationship between factor Ⅻ activity and anticardiolipin antibody in patients of recurrent spontaneous abortion.2 To find some possible results of coagulation factor Ⅻ activity deficiencyMethods: Select 34 non-pregnant patients who had suffered from unexplained recurrent spontaneous abortion as group of RSA ; Select 30 normal non-pregnant women who had at lest one live born child as group of control. All blood samples were detected for anticardiolipin antibody by enzyme linked immunoadsorption assay. At the same time, factor Ⅻ activity of all 64 women's blood plasma were measured by full- automatic coagulation analysis system. (ACL 200, Coulter,American)Chi-square and Fisher's tests were used for statistical analysis. Student's "t" test was used to compare the meansResults: Anticardiolipin antibody were founded in 15 (44.11%) patients from the group of RSA, Of these 3(8.8%) positive, and 12 (35.29%) weak positive. Compare with matched control serum: 6 of 30 (20%), there was a statistically significant difference,(p<0. 05 = .The mean +/-SE factor Ⅻ activity of patients who had suffered fromunexplained recurrent spontaneous abortion was 74.2%+/-37.43%, which was lower than the control group with mean +/-SE of 100.7+/-16.55%. It was a difference of statistically significant. (p<0. 05). Regarding lower than 50% factor Ⅻ activity of standard blood plasma as factor Ⅻ activity deficient, 10 of 34 (29.4%) RSA patients' factor Ⅻ activity deficient, but all the 30 of control group were normal. The difference between two groups had statistically significant, (p<0. 01).In 34 blood samples of RSA, the patients whose anticardiolipin antibody was positive or weak positive had the mean +/-SE factor Ⅻ activity of 72.53+/-49.59%, than anticardiolipin antibody negative patients 74.37+/- 27.21% had a little bit to lower, but the difference had no statistically significant P>0.5).7 of 15(46.67%) anticardiolipin antibody positive or weak positive patients' factor Ⅻ activity was lower, in 19 anticardiolipin antibody negative patients, there were only 3(3/19,15.79%) . The rates of taking place was examined by fisher's test, the difference has no statistically significant, P>0.05. Analyzed by linear correlation, R= -0.17, P=0.925>0.1, hint to the level of anticardiolipin antibody and factor Ⅻ activity in RSA patients have no the linear correlation both.The mean+/-SE APTT in RSA group was 35.26 ± 4.75 seconds , and the data in control group was 36.2 ± 4.38 seconds, the difference had no statistically significant,( P>0.5).In RSA group, the patients whose factor Ⅻ activity was normal had the mean +/-SE APTT of 34.12±4.63 seconds, but the patients whose factor Ⅻ activity was deficient had the mean +/-SE APTT of 38±4.06 seconds. There was a statistically significant difference.(p<0. 05) .we compared the mean+/-SE APTT of factor Ⅻ activity deficiency group and factor Ⅻ activity normal group with that of control group, we have not found a statistically significant difference, P>0.05. hint although the difference in two RSA groups APTT have statistically significant ,but there all belong to the normal scope.In RSA group, the mean +/-SE of fiber proteins was 3.57 ± 0.72g/L,compearwith the control group's 3.33± 0.70g/L, the difference had no statistically significant(P>0.5).In RSA patients, the factor Ⅻ activity deficiency group's the mean +/-SE of fiber proteins (3.74±0.55g/L) was higher than factor Ⅻ activity normal group's, but the difference had no statistically significant( P>0.5).According to the age and the times of spontaneous abortion, we had patients of RSA into difference groups, compared the mean factor Ⅻ activity of each group, we have not demonstrated there was statistically significant difference between... |
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