Cartilage Differentiation Of Rabbit Mesenchymal Stem Cells Induced By Bone Morphogenetic Protein In Vitro And In Vivo

Objective: The trachea plays an important role in the human life for its character in anatomy. The diseases of the trachea may threaten man's life, and surgical therapy is the most effective method for tracheal diseases. A tracheal resection should be carefully considered when the resectional length is more than 6 cm, for the difficulty primary anastomosis and the possible fatal complications postoperatively. So an appropriate substitution is the key point in tracheal surgery. Although artificial prosthesis, autografts, and tracheal transplantation can be used to reconstruct the trachea, the stenosis of the grafts of the trachea is the most critical complication which implicated the reconstruction was unsuccessful. However, the tissue engineering which has been used to resolve some clinical problem on tissue repair brings about a hopeful road to deal with such tracheal diseases. And we have made some progress in tracheal reconstruction, such as the regeneration of cartilage in tracheal grafts, the cultivation of the tracheal epithelium, and the revascularization of tracheal grafts. In this study, we use the mesenchymal stem cells (MSCs) to form cartilage induced by bone morphogenetic protein-2(BMP-2) and supported by poly glycolic acid (PGA), in order to cultivate tissue engineering tracheal cartilage. The final outcome is producing tissue engineering trachea with epithelium to resolve the stenosis post reconstruction. Methods: The MSCs were separated and cultured from rabbit thigh bone marrow.