Expression And Clinical Significance Of Drug-resistant Related Factor In Human Colorectal Neoplasms

Colorectal neoplasms have high morbidity in developed countries. Its morbidity and mortality are increasing these years in our country. The treatment was mainly combine surgery with chemical treatment. But the primary or secondary multidrug resistance (MDR) often influence on effects of chemical treatment. This is the most common and difficult problem. So the resistance of tumor cells becomes focus of medical researches.Ninety-two paraffin embeded primary colorectal carcinoma specimens were examined by P-gP MRP LRP GST- Ï€ and TOPO- II using S-P immunohistochemical methods. And another ten normal specimens were as control. Results were analysed by x2 test. Of the 92 tumor specimens examined 92. 39% (85/92) and 90. 22% (83/92) were positively immunostained for P-gP and TOPO-II, respectively. They were significant higher then the normal ones (P<0. 05, P<0. 01), and the two factors had significant relationship with each other (P<0. 05). 41. 30% (38/92), 84. 78% (78/92) and 97. 83% (90/92) were positively immunostained for MRP, LRP and GST- Ï€ , but they had no statistic significance compared with the normal controls (P>0. 05) . TOPO-II was correlated with patient' s age, the depth of tumor invasion and metastasis of lymph node (P<0. 01, P<0. 01, P<0. 05); There were significant associations between expressions of P-gP and LRP with the depth of tumor invasion. In order to study drug resistance of colorectal carcinomas on gene level, MDR1 genes in tumours and beside tumours were examined by RT-PCR methods. Results were analysed by x2 test. The expression of MDR1 gene in tumours was 80. 56%(29/36), significant higher than that beside tumours 30.56%(11/36) (P<0.01) . The MDR1 expression was positive correlative with the P-gP expression whether in tumours or beside tumonurs (P<0. 01, r₁=0. 7222, r₂=0. 6667) . The match cases of MDR1 and P-gP expression were 86. 11%(31/36) in tumours and...