Effects Of Genistein On Bone Mineral Density And Morphometric Parameters In Orchidectomized Rats

Objective To investigate the effects of genistein on bone mineral density(BMD) and bone morphometry in orchidectomized rats. Methods 40 male Sprague-dawley rats,3 to 4 months old,were randomly divided into five groups: sham-operate(Sham),orchidectomized(Orch),orchidectomized supplied diethyl stilbestrol (DES,5μg/d) or genistein (GenⅠ1mg/d,GenⅡ2mg/d).After 16 weeks with low calcium feed ,the bone mineral density in lumbar spine and femoral neck for all rats were measured by Hologic QDR-2000+DEXA after sacrifice. The bone histomorphometric parameters of left proximal tibia was analyzed by pathology image analysis systems of DMR+Q550. Result (1)BMD: BMD of lumbar and femur of Orch group was lower than sham group(P<0.05). BMD of lumbar and femur of Orch+DES and Orch+GenⅡgroups were higher than Orch group(P<0.05). BMD of femur of Orch+GenⅠgroup was higer than Orch group(P<0.05). (2) Bone hismorphometric parameters: Compared to sham group, TBV/TTV and MTPD were lower and MTPS was higher in Orch group(P<0.05). TBV/TTV and MTPT of Orch+DES group were higher than Orch group(P<0.05). TBV/TTV and MTPT of Orch GenⅠand Orch+GenⅡwere higher than Orch group(P<0.05),but MTPS of Orch+GenⅡwas lower than Orch group(P<0.05). Conclusion Genistein(1mg/d ,2mg/d)and diethyl stilbestrol(5μg/d)can reduced bone loss,increase the number of bone trabecula and reduced the separable degree of bone trabecula on orchidectomized rats.The effect of genistein(2mg/d) on bone is more than genistein(1mg/d).