The Relationship Among Apoptosis Of Peripheral Blood Mononuclear Cells, Related Molecule And The Damage Of Organ Or System In Systemic Lupus Erythematosus

Objective To explore the relationship among the cell apoptosis, related molecule and the pathogenesis of systemic lupus erythematosus (SLE), and to study the influence of the cell apoptosis and related molecule on the organ or system damage in SLE. Methods Flow cytometry (FCM) was applied to detect the early apoptosis rate of peripheral blood monoeuclear cells (PBMC), the expression of the membrane Fas (mFas) and membrane FasL (mFasL) on PBMC. Serum soluble Fas (sFas) and soluble FasL (sFasL) was measured using Sandwich-ELISA. General information and data on the organ or system damage of SLE patients were collected by using self-designed questionnaire containing the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and the Systemic Lupus International Collaborative Clinics/AmericanCollege of Rheumatology Damage Index (SLICC/ACR DI).Results Patients with SLE had a higher early apoptosis rate of PBMC, a higher levelof sFas and a higher expression of mFas compared to healthy controls. The early apoptosis rate of PBMC and the level of sFas had significant correlation with the SLICC/ACR DI in SLE. mFas and sFas also showed a significant correlation with the early apoptosis rate of PBMC. The expression of mFasL on PBMC among patients and controls couldn't be successfully detected by FCM. No significant difference of sFasL level was found between patients and healthy controls (Z=1.32, P=0.195) , and the...