Interleukin-1 Polymorphisms Associated With The Risk Of Gastric Carcinoma In Chinese With Helicobacter Pylori Infection

OBJECTIVE: To evaluate the association between interleukin-1 (IL-1) loci polymorphisms and increased risk of gastric carcinoma in samples from northern Chinese population.METHODS: Blood sample from 76 patients with gastric cancer and 75 controls with chronic gastritis were collected. Genomic DNA was extracted and polymorphisms at -31(C to T), -511 (C to T) and at intron 2 (86-bp VNTR) of IL-lRN were genotyped by PCR-CTPP, PCR-RFLP and PCR.RESULTS: Five kinds of polymorphism of IL-lRN were found as 1/1, 1/2, 1/3, 1/4, 2/2 and the frequencies in patients were 77.63%, 11.84%, 3.95%, 6.58% and 0%, respectively. However, the frequencies in controls were 77.33%, 13.33%, 4.00%, 4.00% and 1.33%. No significant differences were observed between cases and controls in each genotype. The polymorphism of IL-1B-31 allele was C/C, C/T and T/T. The frequencies in patients were 10.81%, 48.65% , 40.54% and the frequencies in controls were 28.38%,48.65% and 22.97%. IL-1B-31 T/T carriers were at an increase risk of gastric cancer with an odds ratio of 4.632 (95%CI, 1.690-12.700). IL-1B-511 alleles were C/C, C/T and T/T. The frequencies in patients were 16.00%, 60.00% ,24.00% and the frequencies in controls were 20.27%,52.70% and 27.03%. No significant differences were observed between cases and controls in each genotype.CONCLUSION: In Chinese population, the polymorphism of IL-1B-31 alleles may be associated with the susceptibility of gastric cancer. However, no evidence was found to support that the polymorphisms of IL-1RN and IL-lB-511 alleles had relationship with gastric cancer.