Study On The Relationship Between SUR1 Gene And Type 2 Diabetes & Blood Lipids

Objective:The sulfonylurea receptor 1 (SUR1) is an essential regulatory subunit of the β-cell ATP-sensitive ( KATP). The possible role of SUR1 gene mutation(s) in the development of T2DM have been reported. Therefore,the purpose of this study is to assess the relationship between SUR1 gene polymorphism and T2DM&blood lipids. Methods:136 normal subjects and 173 patients with T2DM were included. Genomic DNA was extracted from white cells of peripheral blood. The aim fragment containing polymorphism site was amplified by PCR and then was digested by restrictive enzymes.The genotypes were analyzed through the electrophoretogram Sequence analysis was applied to verify the consistency between the genotype and electrophoretogram. SPSS software was used in statistical analysis for the results . Results:1. A silent mutation in exon 31(AGA→AGG,R1273R)was found to show significant difference between T2DM patients and normal subjects. The mutation frequency in exon 31 in patients with T2DM was significantly higher than that in normal subjects (P<0.05);2. In the T2DM patients, sulfonylurea receptor 1 gene exon 31 polymorphism was associated with the level of FINS , BMI, VLDL-C, LDL-C, triglyceride (TG), HOMA-IR and ISI.3. The frequency of "c"allele in intron 24 of SUR1 gene was 59 % in patients and52 % in controls , and that of the "cc"genotype was 33 % and 25 % , respectively. The data was found to show significant differences between T2DM patients and normal subjects.