Protection Effect Of Nerve Growth Factor Against Neuron Injury In Rat Spinal Cord Decompression Sickness

Nerve growth factor, the most typical neurotrophic factors, is one of the most important bioactive molecule which is found earliest. It not only have function of offerting neuron nutriment and promoting nervous process to grow, but also the significance factor of repairing nerve system injury. Clinical experiment research observed that nerve growth factor is closed to some nerve system diseases hard to cure, such as Alzheimer's disease, Huntington's disease, Parkinson's syndrome. But there is no report if NGF has active effect on spinal cord decompression sickness.Decompression sickness involved central nerve system is the fatal cause in chief, without immediate remedy condition at escaping or deeply military diving. Spinal cord injury resulting from decompression sickness, the severe case in diving, is more frequently, which do great harm to diver healthy. For the general DCS, recompression and hyperbaric oxygen have satisfactory curative effect, but infavourable for spinal lesion induced by DCS. Therefore, it is considerable to search for the new method of curing spinal cord lesion induced by DCS for promoting military medical safeguard.SD rats are divided into control group, safe decompression group,spinal cord DCS group, NS group, NGF group, HBO group, NGF and HBO therapeutic alliance group by random in the topic. To observe the expression of NGF, TrkA, TNF- a and neurocyte apoptosis in rats with spinal DCS by IHC and TUNEL, according to the rule, inject NGF into rats by subarachniod cavity in effective time. Compare the expression of TNF- a and neurocyte apoptosis index between NS and NGF group by IHC and TUNEL. Meanwhile, TNF- a is detected in each group by ELISA.Results show as follows:1. There are many congestion and bleeding points, and many small bubbles in spinal cord. Tissue structure is unclear, swell, caryolysis or polynucleation, inflammatory cell infiltrate around blood vessel.2. NGF and TrkA are the important factors in repairing mechanism after spinal cord suffer injury induced by DCS, increasing obviously and coming to peak at 24h. While TNF- a is the impaired factor, enhancing and reaching peak at 48h. It is the same with neurocyte apoptosis.3. TNF- a is obviously less in NGF group than that in NS group at 24h, 48h and 72h by IHC and TUNEL. It is the same with neurocyte apoptosis. Significance difference exists between the two groups (P<0.05).