Partial Exons Of CFTR Mutation Detection And Evaluation Of Its Clinical Significance In Some Kinds Of Male Infertility

Objective: To detect cystic fibrosis transmembrane conductance regulator(CFTR) gene mutation in Chinese patients with congenital biteral absence of the vas deferens (CBAVD) .Severe oligozoospermia and idiopathic azoospermia for the purpose to evaluate whether CFTR gene need be detected before ART or not.Methods: TO choose 25 men with CBAVD, 40 men with severe oligozoospermia, 25 men with idiopathic azoospermia and 40 healthy men, We extracted peripheral venous blood leucocyte DNA and did polymerase chain reaction-single strand conformation polymerphism to detect 12 exons of CFTR gene involving 4, 5, 8, 9, 10, 11, 12, 14b, 15B, 20, 21, 23.Results: There were not CFTR gene mutation involving exons 4, 5, 8, 9, 10, 11, 12, 14b, 15B, 20, 21, 23 in patients with CBAVD, severe oligozoospermia and idiopathic azoospermia.Conclusions: The Chinese patiens with CBAVD, severe oligozoospermia and idiopathic azoospermia were not associated with CFTR gene mutation. CFTR gene mutation is not definitely correlated with etiological factor and spermatogenesis of CBAVD , Severe oligozoospermia...