The Study On The Expression Of Desmoglein 1(DSG1) In Olk And Oscc

Oral leukoplakia (OLK) do not possess the characteristics of any other lesion that can be defined , with white lesion on the oral mucosa, and Some can be turned into the cancer. The unusual hyperplasia of the mucous membrane is a essential feature of the precancer ,which is the transform stage from normal conditiont to malignan cancer. It is much stages , many steps from OLK to OSCC. Recent datas show that the expression of desmosome reduces in tumour, even no expression in malignant tumour especially.Desmosome play a certain role in the tumour cancerate and transformation. So we adopted immunohistochemical technique to study the expression of DSG1 in oral normal mucous ,OLK mucousa , OSCC mucousa and oral epithelium cultured in vitro.ObjectivesTo study the expression of Gsg1 in oral leukoplakia, and discuss the mechanism of the transformation from oral leukoplakia to carcinoma.Material and methods1. The expression of DSG1 were examined with immmuhistochemical SP method in the oral epithelium cultured in vitro, through regulating the density of calcium particle in medium. The primary OLK fibroblast was obtained by tissue culture. Drawing growth curve and measuring biological vigor by MTT method between OLK fibroblast and normal fibroblast .And the expression of cytokeratin, vimentin were examined with immmuhistochemical method .2. Tca—8113 cocultured with NFs, OLK fibroblast in the same condition for threedays. The expression of DSGl were examined with immmuhistochemical SP method inTca-8113.3. The expression of DSGl were examined with immmuhistochemical SP method in Paraffin wax sample which were from Shandong University stomatologicalhospital from 1995— 2002,including 10 normal oral mucous,35 OLK mucous(17 simple hyperplasia , 9 mild dysplasiarespectively,6 moderate dysplasiarespectively,3 severe dysplasiarespectively)and 30 oral squamous cell carcmomas(OSCC 112, OSCCII 10, OSCC III 8). And a half quantitative analysis was carried with the result ,and dealed with statistics.4. The changes were observed in different stage of canceration in OLK by transmission electronmlcroscope.Results1. We obtained oral epithelium and OLK fibroblast cultured in vitro. In the general ,the growth speed, proliferation and mitosis ability and vitality of the OLK fibroblast and NFs(p<0 .05). The oral OLK Fs showed negative staining for cytokeratin, and positive staining for vimentin .2. Result of EH: In normal oral mucosa, the positive rate of DSGl was 100%, and 94.1% in simple hyperplasia OLK, 27.8% in atypical hyperplasia OLK,13.3% in OSCC. There was positive result when the density of calcium particle in medium was 1.0 mM. There was negative result in Tca-8113,and there was positive result in Tca-8113 cocultured by OLK fibroblast.3. Result of OD: OD shrinked in order in in normal oral mucous, simple hyperplasia OLK, atypical hyperplasia OLK, OSCC. There were significant difference between each group(p<0.05).In OSCC, There were significant difference between each group(p<0.05).4. Result of transmission electronmlcroscope: In normal oral mucous there were plenty of desmosome with plenty of keratin intermediate filaments joined ,and nucleus was not destroyed. In canceration normal structure of desmosome weredestroyed, and keratinintermediate filaments were curled like balls, and nucleus was destroyed. ConclusionThe study shown that expression of DSG1 reduced in OLK especially in dysplasiarespectively OLK, and expression of DSG1 reduced more than OLK in OSCC ,and there is on expression in OSCCIII. This suggested that it play a role in the stage of OLK transferming to OSCC. This provides a new way for treating OLK and interdicting OLK cancerization.