Expression And Significance Of S100A4,E-cadherin And Nm23-H1 In Cervical Squamous Carcinoma

Invasion and metastasis are hallmarks of malignant tumors. The activation of many genes and the expression of their products have been shown to be important in tumor progression. Cervical cancer is one of the most malignant diseases and a serious threat to women's health. Although the synthetical therapy of the cervical cancer was enhanced, the death rate was still the second . Invasion and metastasis are the most important prognostic fectors of the cervical cancer. To prolong the life of the cervical cancer's patients, the pure surgery and the lack of special actinotheraphy and chemotherapy are not enough. To provide reliable indicators of prognosis or aggression or therapeutic significance for cervical cancer, we need go on researching the etiology and pathology .Throughout the past few years, the S100 family has emerged as an important group of proteins with the capacity to promote invasiveness and metastasis of many human neoplasms. In particular, recent studies shed light on the mechanisms of action of protein S100A4, and indicate its possible prognostic role in human neoplasms by an analysis of online serial analysis of gene expression (SAGE) database. In order to clarify the molecular mechanism of human Cervical cancer , we collected specimens from cervical squamous carcinoma and normal cervixes. The SP immunohistochemical method was used to detect the expression of S100A4 E-cadherin and nm23-Hl in 65 cases of cervical squamous carcinoma and 15 cases ofnormal cervical tissues. It will provide theoretic basis for tumorigenesis mechanism and gene therapy.Materials and methods:80 cases of paraffin wax embedded samples were available from the First Mfiliated Hospital of Zhengzhou University. Of them 15 were normal cervical tissues and 65 were cervical squamous carcinoma tissues. Of the Cervical cancer specimens , there were 17 in stage I , 39 in stage II, 8 in stage III, 1 in stage IV according to the guidelines of the International Federation of Gynecology and Obstetric(FIGO). According to their histological grading, the cases of Cervical cancer were divided into three groups: 21cases of grade I, 34 cases of grade II, 10 cases of grade III. Of the Cervical cancer specimens , 24 accompanied with lymph node metastasis. To elucidate the involvement of S100A4, E-cad and nm23-Hl in cervical squamous carcinoma, we examined expression of the protein by immunohistochemistry using monoclonal antibodies against E-cad , nm23-Hl and polyclonal antibodies against S100A4. Statistical analysis was performed by the SPSS 10.0 software package. Differences among groups were analyzed by using analysis of the Chi-squared test and Person Correlation. All of the P values resulted from two-sided statistical test. Statistically significant level was considered as "alpha equals 0.05"( a =0.05).Results:1. The expression of S100A4 was negative in the normal cervical tissue; it wasdetectable in cytoplasm or/and membrane in the cervical cancer tissue. There was also positive expression in smooth muscle of blood vessel. The positive expression rate of S100A4 was 35.4% (23/65 ) in cervical squamous carcinoma. There were significant differences between cervical squamous carcinoma and normal group(P<0.001).2. The positive immunostaining rate of E-cadherin in normal and cervical squamous carcinoma were 100%(15/15) and 33.8%(22/65) respectively. There were significant differences between cervical squamous carcinoma and normal group(P <0.05).3. The positive immunostaining rate of nm23-Hl in normal and cervical squamous carcinoma were 10O%(15/15) and 44.6%(29/65) respectively. There were significant differences between cervical squamous carcinoma and normal group(/J <0.05).4. The expression of S100A4 positively correlated with the clinical stages and lymph node metastases (P <0.05), but not with the differentiation of tumor (P >0.05 ) ; E-cadherin expression correlated with lymph node metastases and the differentiation degree of tumor(/?<0.05), but not with the clinical stages(/?>0.05). nm23-Hl expression correlated with lymph node metastases and the differentiation degree of tumor (P <0.05), but not with the clinical stages(