Relationship Study Between Macrophage Infiltration,VEGF-C Expression And Larygneal Squamous Cell Carcinoma

Objective:To evaluate the infiltration of Macrophage and expression of vascular endothelial growth factor-C (VEGF-C) in laryngeal squamous cell carcinoma, to study their effect on laryngeal carcinoma and lymph nodes metastasis. Methods:68 cases of laryngeal squamous cell carcinoma and 20 cases adjacent safety margin were examined by immunohistochemical SP method using monoclonal antibody to CD68 and VEGF-C , All statistical analyses were performed by SPSS13.0. Results:(1) Macrophage counts in stroma of laryngeal squamouscell carcinoma were higher than in parenchyma for 68 cases, and were higher than that of 20 cases of adjacent safety margin.(2) macrophage counts in pathological grade Ⅰ , clinical grades T1+T2, and non-metastasis of regional lymph node were obviously lower than those in cases of pathological grade Ⅱ, Ⅲ , clinical grades T3+T4, and metastasis of regional lymph node.(3) VEGF-C protein expression was significantly higher in laryngeal carcinoma tissue than in normal tissue. VEGF-C expression in laryngeal carcinoma of pathological grade Ⅰ , clinical grades T1+T2 were lower than pathological grades Ⅱ, Ⅲ ,clinical grades T3+T4. VEGF-C protein expression was significantly higher in lymphatic metastasis group than in non-metastasis group.(4) There was a negative correlation between VEGF-C expression and counts of Macrophage in stroma (r=0. 599, P = 0.01) and there was a positive correlation between VEGF-C expression and counts of Macrophage in parenchyma (r=—0.526, P=0.007) .Conclusions:(1) Macrophage infiltrated within the laryngeal squamous cell carcinoma tissues of stroma and parenchyma maybeserve as two roles: macrophages in parenchyma had the anti-tumour immune response ; but those in stroma may potentially supported the angiogenesis ^ metastasis and prognosis of laryngeal squamous cell carcinoma .(2) These findings provide evidence supporting the involvement of VEGF-C expression in the promotion of lymph node metastasis in laryngeal squamous cell carcinomas. Furthermore, examination of VEGF-C expression in biopsy specimens may be beneficial in the prediction of cervical lymph node metastasis and prognosis of laryngeal carcinoma.(3) There was obvious correlation between Macrophage infiltration and VEGF-C expression. They may serve as coordinating function in lymph node metastasis of laryngeal carcinoma.