The Relationship Of Expression Of Vascular Endothelial Growth Factor A Between Lymphangiogenesis And Angiogenesis And Lymph Node Metastasis

Objective: 1,To investigate the expression of vascular endothelial growth factor A (VEGF-A) in human epithelial ovarian carcinoma. 2,To investigate the relationship of vascular endothelial growth factor A (VEGF-A) between Lymphatic vessel density(LVD) and Microvessel density (MVD) and lymph node metastasis in epithelial ovarian carcinoma.Our target is to explore the role of vascular endothelial growth factor A (VEGF-A) in Lymphangiogenesis and Angiogenesis of ovarian carcinoma. Methods: 1,Immunohistochemistry was used to investigate the expression of VEGF-A in 59 samples of ovarian carcinomas, 20 samples of borderline ovarian tumors ,42 samples of benign cystadenomas. 2,The expression of vascular endothelial growth factor receptor 3 (VEGFR -3) proteins in ovarian carcinoma was detected by Immunohistochemistry,as well as the expression of CD34 proteins in epithelial ovarian caicinoma.LVD and MVD was counted by manual counting.The relationship of vascular endothelial growth factor A between LVD and MVD and clinicopathological characteristics was analyzed. 3,The relationship of clinicopathological characteristics between LVD and MVD was analyzed. 4,The expression of the mRNA of vascular endothelial growth factor A (VEGF-A mRNA) in 31 ovarian carcinomas and 22 benign cystadenomas was detected by Reverse transcriptase polymerase chain reaction (RT-PCR).The results were analyzed by gel imaging system. 5,The relationship of VEGF-A mRNA between LVD and lymph node metastasis was analyzed. Results: 1,The expression of VEGF-A were both expressed in ovarian carcinomas, borderline ovarian tumors and benign cystadenomas.The expression of VEGF-A in ovarian carcinomas was significant higher than that in borderline ovarian tumors( P<0.05). The expression of VEGF-A in borderline ovarian tumor was significant higher than that in benign ovarian tumor( P<0.05). 2,MVD was significantly higher in the group of VEGF-A positive expression than that in the group of VEGF-A negative expression ( P<0.01). also does the LVD( P<0.05).VEGF-A was positive relevant to lymph node metastasis ( P<0.05),clinic staging( P<0.01) and peritoneal metastasis( P<0.05) 3,Positive staining of VEGFR –3 was located in ovarian carcinoma cells and endothelium cells of vessel.LVD was significantly higher in the group of positive lymph node metastasis than that in the group of negative lymph node metastasis ( P<0.01). also does the later clinic staging( P<0.05) and positive peritoneal metastasis( P<0.05).MVD was significantly higher in the group of positive lymph node metastasis than that in the group of negative lymph node metastasis ( P<0.05), also does the positive distant metastasis ( P<0.01) and later clinic staging( P<0.05) and positive peritoneal metastasis( P<0.05). 4,The expression of VEGF-A mRNA in ovarian carcinomas was significant higher than that in benign ovarian tumor( P<0.001). LVD was significantly higher in the group of VEGF-AmRNA positive expression than that in the group of VEGF-AmRNA negative expression ( P<0.05). There was a positive correlation between the expression of VEGF-AmRNA and lymph node metastasis ( P<0.05). Conclusion: 1,The expression of VEGF-A was up-regulated in varian carcinomas. MVD was significantly higher in the group of VEGF-A positive expression than that in the group of VEGF-A negative expression ( P<0.01). also does the LVD ( P<0.01).The expression of VEGF-A appears involved in the promotion of Lymphangiogenesis and Angiogenesis in epithelial ovarian carcinoma 2 Blocking the role of VEGF-A could be helpful to ruduce the dangerous factor oftumour Because of the role of VEGF-A in Lymphangiogenesis and Angiogenesis, which is expected to be a new effective treatment method of tumour.