| Objective To observe the changes of VEGF (vascular endothelial growth factor) GLUT1 (glucose transporter 1) released by cultured retinal M(?)ller cells in high glucose high insulin and Tangwang I solution in vitro ,to study the mechinism of Tangwang I in DR (diabetic retinopathy) .Methods 1. To procedure the primary culture of retinal M(?)ller cells with suspended constitution. 2. To detected the cellular activity of cultured rabbit retinal Muller cells in Tangwang I solution at different concentration under high glucose , to determine 50% cethal dose. 3. Immunohistochemistry and flurimetry methods were used to assay the changes of VEGF GLUT1 released by cultured retinal M(?)ller cells in vitro in high glucose high insulin and Tangwang I solution.Results 1. The cellular activity can be decreased by high glucose, which was inhibited by Tangwang I . 2. The expression of VEGF of retinal M(?)ller cells in vitro was enhanced dramatically by high glucose and high insulin.It was expressed mostly on the third day,while it expressed least on the fifth day. 3.The expression of GLUT1 of retinal M(?)ller cells in vitro was enhanced significantly under highglucose and high insulin on third day, and membrane of cellular organelle mainly expressed.4. Tangwang I can decrease the expression of VEGF and GLUT1 of high glucose solution.Conclusion 1. The cellular activity can be decreased by high glucose, which was inhibited by Tangwang I . 2. The expression of VEGF of retinal Muller cells in vitro was enhanced dramatically by high glucose and high insulin.It was expressed mostly on the third day,while it expressed least on the fifth day. 3.The expression of GLUT1 of retinal M(?)ller cells in vitro was enhanced significantly under high glucose and high insulin on third day, and membrane of cellular organelle mainly expressed. 4. Tangwang I can cure the DR by decrease the expression of VEGF and GLUT1 of high glucose solution. |
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