Study On Embolization Of Renal Arterial Of Rabbits With Fibrin Sealant And Gelatin

Objectives: To investigate the property, safety, effectiveness of the embolization of renal arterial of rabbits and the reabsorb time with Fibrin sealant embolism (FSE) and gelatin embolism (GE) by transcather.Materials and Methods: The FS was made into segments of 2cm embilization material. In vitro tests, and is delivered through different catheters and pushed by guide wire or sodium chloride injection. To observe the FSE and GE resolved effect by the urokinase. To investigate the releasing level of methylene blue contained in the FSE and GE. To study the coagulant level of the FSE and GE. In animal experiments, sixteen healthy rabbits were divided into experiment group and control group at random. The experiment group had been embolized with FSE. The control group had been embolized by GE. After the embolization, at instantly, 10mins, 1d, 3d, 1w, 2w, 3w, 1m, 2m, angiography was respectively processed to observe what had happened in renal artery. Artery blood was drawn from each rabbits before the test and 1d, 3d, lw, 2w, 3w, 1mon, 2mon after embolization for examinations of liver and renal functional changes. 1d, 3d, lw, 3w, 2mon after the embolization, the animals were respectively killed to obtain specimens of embolized kidney to observe the histopathology changes. The renal artery of rabbits had been embolized with FSE and GE. After 1 hour, urokinase were injected into the renal artery and study the artery effect of recanalzation.Results: In vitro tests and studys appeared that FS could be delivered more easily than GE. The FSE released from 4F to 7F catheter could be easily pushed by guide wire or sodium chloride injection. Whether in vitro tests or animal experiments, the FSEcould be resolved by urokinase. But the GE couldn't be resolved by urokinase. The methylene blue contained in FSE could be slowly released into sodium chloride injection. The FSE had stronger coagulant ability than the GE. In animal experiments, the FSE had as well embolic effectiveness as the GE at the first week. The FSE was reabsorbed after 1 week, but the GE was reabsorbed after 3 weeks. The FSE and the GE had the same recanalzation rate(P>0.05=0.619). The pathology experiment revealed that the FSE had less impairment to the kidney than the GE. Whether been embolized with the FSE or GE, no significant difference (P>0.05) was observed on the changes of the liver function and renal function of the rabbits.. Conclusions:1. FSE is an effective, safe, useful, easy-made and easy-released targeted embolism.2. FSE containing drugs can be slowly released, this is valuable for the further study.3. FSE can be applied in clinic as a short time or mid-time embolic agent. It is new good material of embolic agent as well as GE and has good value in clinic. Both of the FSE and GE can embolize renal artery at first week, but FSE can be reabsorbed after 1 week.4. GE is a mid-time embolic agent and recanalzation time is about 3 weeks.5. FSE can be resolved by urokinase, which can be used as a rescue method when mis-embolism occurred, or make embolized vessel recanalzated to lessen impairment of tissue.