| OBJECTIVE: To investigate the expression of five Cancer-testis antigens(CTA) mRNA in human glioma, and determine the potential of these CATs for immunotherapy of glioma.METHODS: Seventy-five specimens of human glioma and eleven specimens of relatively normal tissues from human brain were analyzed for the expression of five CTA genes with Reverse Transcriptase Chain Reaction (RT-PCR). Correlation between the expression of CTA genes and clinical data was evaluated.RESULTS: NY-ESO-1 was negative for all of glioma tissues tested.The expressive frequency of NY-SAR-35 in glioma was 22.67% ( 17/75) .The expressive frequency of SSX in glioma was 33.33% (25/75) , in which the expressive frequency of SSX1 was 5.33% (4/75) , SSX2 and SSX4 was negative for all specimens tested. There was no statistic significance for the expression of NY-SAR-35 and SSX1 correlated with patients'sex, age and clinical pathological grade of glioma (P>0.05). Of 11 relatively normal tissues, all of cases negatively expressed for five CTAs tested except one case expressed SSX and SSX2.CONCLUSION: With expressive frequency in glioma, SSX1 and NY-SAR-35 might be a potential target for immunotherapy of glioma. However, only two of five CTAs tested expressed in glioma, further looking for more tumor-specific antigens with a frequent expression in gliomas is warranted for glioma immunotherapy. |
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