Extraction Of The Genomic DNA From Osteosacoma And Partial Digestion Anylisis

Background Osteogenic sarcoma is the most common primary malignant tumor of bone, with an incidence of 2-3 cases per 1,000,000 populations. It usually occurs in the distal femoral metaphysis, proximal tibial metaphysis, proximal humeral metaphysis, pelvis, and proximal femur. Most patients are in the adolescent age group. The natures of this tumor are highly malign and easy metastasis. In recent years, many routine therapies, such as neoadjuvant chemotherapy have been debeloped, limb salvage surgery. The 5-year survival rate currently can be reached to 60%-80%, but a few patients are dead from local recurrence and metastasis. Aimed to resolve Osteosarcoma and lung metastases, people invested and further studied many good-future tumor gene therapy methods such as immune gene therapy, antisenes gene therapy, suppressor gene therapy, suicide gene therapy and so on. Osteosarcoma gene therapy has been made a certain progress, even some gene therapy plan has show clinical experimental plan, but present work only lies in experimental phase , many key techniques and theory need further research too.Objective (1)To prepare the products of partial digested genomic DNA suitable to be ligated to EMBL3. To accomplish primary work for constructing Osteosarcoma genomic DNA bibrary with EMBL3 vector;(2) In order to develop a simple , speedy, reliable and inexpensive method for extracting high molecular weight genomic DNA, comparing several methods of extraction of genomic DNA from Osteosarcoma.Methods CDBy conventional phenol-chloroform extraction method and improved silver stick twist, genomic DNA from Osteosarcoma was extracted , the quality of extracted genomic DNA was identified by mensurating the A260 and A280 and analyzed by electrophoresis. (2)By...