| Objective: Syphilis is a chronic, systemic and infectious sexually transmitted disease caused by the Traponema pallidum infection. It results in multiple clinical symptoms and hurts most of the organs in the body. The incidence of syphilis gradually increased in the recent years and about 20,000 new cases of syphilis occur annually. The epidemiological study proved the relationship of syphilis and the human immunodeficiency virus (HIV) that syphilis enhance the risk of transmission and acquisition of HIV. Early, rapidly and exactly diagnosing syphilis play a significant role on eliminating infection sources and cutting the route of transmitting through people, because infection source of syphilis is the syphilis patient. In the present, the ways of the clinical tests for syphilis include direct tests of T.pallidum and serologic tests. The direct tests including dark field microscopy examination or direct fluorescent antibody technique, are usually affected by using medication, the site of lesions and the operator's experience. The serologic tests detect antibody against T.pallidum. Tests yield an effective result after the body comes into being antibody as a result of infection. So serologic tests can delay to discover infection and increase epidemic chances. Nanotechnology is a new technology that has been developed rapidly and applied widely. It utilizes the particular properties that appear due to the superficial, small dimension and quantal tunnel effects of nanoparticle. For the specialties, we can combine nanoparticle with protein, antibody, DNA and so on. So nanoparticle becomes a kind of non-radioactive marker. The nanoparticle marker has lots of characters, including that marker way is easy and convenient, by physical binding affinity matter of high molecule (for example protein, antibody) don't change, by using different sizes of nanoparticles we can put up multi-mark, the detection of using nanoparticle only need a very small quantity of sample, immuno-nanoparticle is inactive so we can preserve them for ages, and it is short by using nanotechnology so on. The purpose of the investigation is detecting antigen of T.pallidum by using nanoparticle and lead nanotechnology in early diagnosing syphilis. This will provide beneficial explorations for setting up the new method of detecting T.pallidum in screening and identification. Methods: 1. Detecting different nanoparticles by using violet-ray light and fluoroanalyzer; 2. Preparing specific nanoparticle by using the fluorescent chelate nanoparticle that is coated with a monoclonal anti-TP antibody, and observing specific nanoparticle solution by using violet-ray light and fluoroanalyzer; 3. Detecting antigen of T.pallidum by observing the changes of the compound under violet-ray light and fluoroanalyzer. Results: 1.We can observe different colorful fluorescences of different size of nanoparticles by macroscopic observation under violet ray of identical wavelength and different position and intensity of fluorescence by using fluoroanalyzer; 2. The specific nanoparticle solution is stable, clarity, and the blue shift of fluorescence by macroscopic observation under violet ray light; We can observe that the position of the fluorescence of this solution moves about 10nm to left by fluoroanalyzer and the intensity decreases; 3. The fluorescent sediment and non-fluorescent supernate fluid in the compound of specific nanoparticle and antigen of T.pallidum can be observed under violet-ray light; The fluorescence in...
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