The Protection And Mechanism Of DMSO To Cytotoxic Caused By Depleted Uranium

ObjectiveThe aim of this study was to explore the protection effect and mechanism of DMSO on cytotoxic, genotoxic, clastogenic and mutagenic which caused by depleted uranium and offer the gist of defending DU damage Methods:The immortalized human bronchial epithelial cells (BEAS-2B) were used to study the cytotoxic potency induced by DU, the productions of ROS were detected in BEAS-2B cells exposed to DU. Spectrometric method and fiuorometric method were used to measure the intracellur and extracellular production of superoxide anion, hydrogen peroxide and hydroxyl rasical, activity of anti-oxidant enzyme reduced by DU, cell morphological change and molecular alteration of the transformed cells, escaping of intracecellur enzyme and activity of ATPase to be proved the damage of cell membrane. All of these is to be researched the the protection and mechanism of DMSO to cytotoxic , genotoxic, clastogenic and mutagenic which caused by depleted uranium.Through observing comet assay and mutation frequency of hprt gene, frequencies of Micronucleated and Multinucleated Cells .chromosome analyse, we research the genotoxic induced by DU and protection of DMSOResults:1 , The ROS production including H₂O₂,O₂ "and OH , in DU induced BEAS-2Bcells increased remarkably ,and these change could be inhibited effectively by0.5% of DMSO. 2, DU induced cell DNA single breaks, tail area, tail DNA% tail lengthtail moment , olive tailmoment increased significantly and these incrementscould be inhibited effectively by DMSO 3 , The frequencies of micronucleated and multinucleated cells in BEAS-2Bcellswere significantly increased by DU and these increments of micronucleated cellscould be inhibited effectively by DMSO 4, The mutation frequency of hprt gene were significantly increased by DU and theincrements could be inhibited effectively by DMSO 5 , The G-banding technique was used to analysis the metaphase modal chromosome...