Full-Lengh Cloning Of Graves Disease Differentially Expressed Gene And Bioinformatical Analysis

Objective To obtain full lengh cDNA of Graves disease differentially expressedgene (TGDRF12) by SMART Rapid Amplification of cDNA Ends in the foundation of low-grade expressed sequence tags (CF569048) screened by suppression subtractive hibridization.Methods Graves disease differentially expressed gene fragment ,TGDR12(GenBank accession number:CF569048) is especially low-grade expressed sequence tags screened by suppression subtractive hibridization. After bioinformatical analysis and obtained 5'RACE and 3'RACE of cDNA of TGDR12 by SMART Rapid Amplification of cDNA Ends. Then removing vector sequences from raw sequences and assembled the full cDNA. The sequence was analyzed in GenBank by using BLAST programs (http://www.ncbi.nhl.gov/ Blast) about its open reading frame and biologic functions.Furthermore,test its expressed in multiple tissues and organs of nomal human by using Multiple Tissue Northern Blot .The novel full sequence was submitted to GenBank by sequin program (http:// www. ncbi. nhl. gov/Sequin).Results We had obtained full lengh cDNA of Graves disease differentiallylow-grade expressed gene by SMART Rapid Amplification of cDNA Ends .The cDNA sequence of it is 1620bp in size ,the open reading frame is 318bp and capable of encoding 106 amino acids. The 5'-untranslated region is 78bp, while the 3'- untranslated region is 1224bp.It expressed in multiple tissues of nomal human by using Multiple Tissue Northern Blot and analysis showed that TGDRF12 had only one transcripts (1.6kb) and expressed highly in stomach , thyroid and adrenal gland, especially in thyroid. The sequences was analyzed in GenBank by using BLAST programs indicated it has highly homogeneity with the family of protein arginine N-methyltransferase(PRMTs), probably it is a novel one in the family of PRMTs. It had accepted in Nr Database of NCBI ( accession...