Study On Contents And Speciation Of Arsenic In Algae And Metabolize Of Arsenic In Mice

The four common algae. Laminaria japonica , porphyra , Undaria pinnatifida and Sargassum fusiforma, all contain abundant calcium, iron, phosphorus, iodine and other substance. They can promote the development of skeletons, and are high nutrient value seafood which have health-care function. Nevertheless, algae have function of enriching arsenic in seawater. These algaecontain plentiful poisonous arsenic element, which can cumulate in vivo and result in latent threat to human health after they enter human body. Therefore, contents of arsenic must be detected in good time and controlled effectively in course of exploit and machining of seafood.Secondly, arsenic bring about attention of more and more people because of their endangerment to human health. Mechanism on metabolize and carcinogenicity of arsenic in human body has been a hotspot problem which was concerned by scholars from many country. at the same time, although arsenic is a poisonous substance, there are reports on curing acute promyelocytic leukemia and chronic myeloid leukemia patients with arsenite acid injection, and has good curative effect. Consequently, the research on metabolize and biotransformation of arsenic compound in vivo has important significance.The experiment was conducted surrounding two aspect,1. Analysis of contents and speciation of arsenic in some algae. Samples were prepared with dry digestion process to determine the total arsenic contents, and determination of contents adopted Silver Salt Spectrophotometry Method and Hydrid Generation-atomic Fluorescence Spectrometry Method respectively. The conditions of determination which include the concentration of KBH₄, effect of reducing agent and concentration of HC1 medium were discussed, and the two determination method were compared. Samples were extracted with HC1 solution for 18h to determine the inorganic arsenic contents with Hydrid Generation-atomic Fluorescence Spectrometry Method. Results showed, campared with Silver Salt Spectrophotometry Method, determination of contents of arsenic with Hydrid Generation-atomic Fluorescence Spectrometry Method is more sensitive, precise, easy and reliable. The linearity of total and inorganic arsenic was great, correlation coefficient is above 0. 9990. The four algae have high arsenic levels, are all above 16. 58μg·g^-1. The inorganic arsenic contents are also high, and have great difference. Sargassum fusiforme has the highest totaland inorganic arsenic contents in these algae, are respectively 64. 89|j.g? g1 fP 45. 53|ig? g1; the distribution of inorganic arsenic has region difference. This paper provides a reliable method to study the distribution feature of arsenic contents of algae systemically. Capillary electrophoresis method was established to analyze As (III) ., As(V) and DMA in algae. The electrophoresis condition, linearity range, limit of detection and sample pretreatment method were discussed. Although no As(III)> As(V) and DMA in algae were detected, it provided reference foundation for speciation analysis of arsenic in algae later, and for determination of arsenic in other samples.2. Study on metabolize of arsenic in mice with GC method. GC/MS and GC were adopted to detect As (III) respectively, and find out sample pretreatment method of As (III) in plasma and red blood cells. It was established determination method of As (III) in plasma and red blood cells, and metabol ize of As (III) in mice blood was studied according to this method. Concentration-time curve and distribution of As (III) in plasma and red blood cells were described after intravenous administration with dose of 5mg*kg"'. The pharmacokinetics parameter of As (III) were gained through software finally.