Study On The Maternal-Fetal Transmission Of Rubella Virus By Quantitative PCR

Objective: Can gravida who infection RV convection virus to their fetal ? whether have to harvesting artificial abortion ? this is a problem which clinicle are faceing for many years . the investication is studying on the quantitative relationship of the Rubella Virus(RV) loads between the RV-infected gravidas and their infants, try to provid some scientific evidence for prevent CRS.Method: The levels of RV-RNA in the serum samples of 23 pou/couples of infants and gravidas, who were detected as RV-IgM antibody positive, were exmained by the fluorogenic quantitative real-time PCR,to make the auto-control between morthers and infants.Result: The RV-RNA loads in the serum of the gravidas was positive correlated with that of the infants. When RV-RNA loads in the serum of the gravida is about 7.93×10~2 ± 2.03×10~2 copies/μL, RV could not be detected in the serum of the infants. When RV-RNA loads in the serum of the gravida is about 3.89×10~3± 1.10×10~3 copies/μL, RV-RNA loads in the serum of the infants is about 2.45×10~3 ± 1.01×10~3 copies/μL , When RV-RNA loads in the serum of the gravida is about 7.39×10~3± 1.85×10~3 copies/μL, the RV-RNA loads in the serum of the infants is about 5.99×10~3±1.30×10~3copies/μL. And the levels of RV-RNA in the serum of the infants incresased with that of the gravidas.Conclusion: The RV-RNA loads in the serun of the gravidas and that of the infants have a positive relationships. The level of 3.89×10~3±1.10×10~3copies/uL of the RV RNA in the serum of the gravida maybe could to be used as the threshold to judge if a gravida could transmit RV to her infant during pregnancy. There are some values to clinics in themry and practic.