Effects Of Ox-LDL Damage On Erythrocyte

With the development of hemorheology research,investigates in the mechanism of oxidative membrane damage at the cellar and molecular levels would be helpful to exploring the physiologic and clinic sequence in the changes of erythrocyte deformability and offering important theories to basic pathology and clinic research of diseases correlated to erythrocyte. It is generally accepted that LDL was oxidative damage to become Ox—LDL, Ox—LDL damaged endothelium which was changed foam cells then formed AS focus, which means Ox—LDL promoting to occur AS. Author has investigated a lot of academic literature and founded few knowledge on Ox—LDL oxidative damage erythrocyte. Experiments results indicated that there are significant regression relations between the membrane viscoelastic properties and the oxidative damage mechanism. The regression relations suggested that oxidative damage reaction result from the superoxide anions may be same or similar biomolecular mechanical induced the changes of membrane viscoelasticity and elastic and deformability of erythrocyte. The experimental results suggested that erythrocyte membrane protein oxidative damage reaction may be an important molecular mechanism inducing changes of membrane viscoelasticity and elastic even of whole cell deformation. Of the many factors that affect erythrocyte, our objective was to determine whether Ox-LDL has an effect on t the erythrocyte. In this research work, the effect of Ox-LDL oxidative damage on membrane viscoelasticity was investigated with micropipette aspiration method, applying foundamental and methods of cell rheology, in the theory analysis of erythrocyte deformation by using the Chien "hemispherical cap model", we used D_p/(R_p/Δp)～(t-t1)/τcurve to estimate the effect of different oxidative damage extents on the time-dependent and Δp-dependent deformation course of erythrocyte into micropipette; The molecule biology experimental results indicated that the oxidative damage led to a decrease in the contents of the membrane protein. SDS-PAGE scanning of the erythrocyte membrane protein showed that a new high molecular weight protein component appears before the spectrin1,2 and some lower molecular weight protein bands appear between spectrin1,2 and band3. The % spectrin1,2,B4.1,B4.2 and lower molecular bands had decreased. The molecule biology experimental results indicated that erythrocyte membrane oxidative damage has a great influence upon the contents of the membrane protein meanwhile the experimental results suggested that erythrocyte membrane protein oxidative damage reaction may be an important molecular mechanism inducing changes of membrane viscoelasticity and elastic even of the whole cell deformation. Deformability is a very important property of the erythrocyte. It is generally accepted that the membrane of an erythrocyte is important in determining its deformability not only because the membrane mechanical properties and surface area are dominant factors, but also because membrane transport affects the cell internal viscosity. Based on our experiments, we may conclude that Ox-LDL mainly damaged erythrocyte membrane which made change the contents of the membrane protein and reduced the ability of deformation ,carry oxygen and release oxygen. The reducing of erythrocyte deformation ability lead to blood viscosity elevate ,velocity of blood flow slow down and the shape of erythrocyte changed .All the factors make endothelium damaged and blood platelet activated. So, the descending of erythrocyte deformation is not the original factor but a succeeding factor to AS and anticipate the expansion of AS.