Studies On The Quality Standard Of The Compound Dandelion Enema

Chromatographic fingerprint, a novel technique for assessing the quality of herbal medicinal products, is widely accepted at present. In this study, several kinds of analysis apparatus were used to establish a fingerprinting method for the control of the quality of the compound dandelion enema and its crude medicinal materials. The method which combined a qualitative fingerprint of its ethyl acetate extract with a quantitative measurement of the effective components could be developed as a modern criteria for accurate control of the quality of the compound dandelion enema.This thesis includes seven parts. In the first part, the characteristics of fingerprint spectrum of Chinese traditional medicines were reviewed, and the diffrernt analysis methods of establishment of fingerprinting spectrum of Chinese traditional medicines were introduced, At the same time, the chemical composition, pharmacological activities and clinic applications of the compound dandelion enema and its crude medicinal materials were also summeryed. In the second part, ethyl acetate was tested to be the most suitable extracting solution. Mass-to-charge ratios (M/Z) of the compound dandelion enema and its main crude medicinal materials were obtained by ESI-MS and the main components of compound dandelion enema were detected and assigned by this method. The results showed that the effective components in the samples were mainly from Taraxacum mongolicum Hand.-Mazz, and it was significative to study the quality standard of the compound dandelion enema by investigating Taraxacum mongolicum Hand.-Mazz.In the following experiments, different kinds of analysis apparatus were used to study the fingerprint of the compound dandelion enema. Firstly, the compound dandelion enema and its crude materials were identified according to the R_f value,color and demension of the spots on their thin-layer chromatography (TLC) and with the fingerprinting analysis of the entire chromatography, and the layer chromatographic parameters were achieved bydigital photograph and TLC scans. The results indicated that some known compounds were identified as caffeic acid, protocatechualdehyd. Seconddly, a high performance capillary electrophoresis (HPCE) method was utilized to separate caffeic acid and ferulic acid from the compound dandelion enema and its crude material, and establish a method of the determination of the content of caffeic acid. The results showed that the method was simple, rapid, sensitive and could be used to control the quality of the compound dandelion enema. Thirdly, a qualitative and quantitative RP-HPLC method was established with fingerprinting technology for the control of the quality of the compound dandelion enema and its crude material. The HPLC fingerprinting was established from 10 batches, and the data showed there were 23 characteristic peaks in the compound dandelion enema and 11 specific peaks in Taraxacum mongolicum Hand.-Mazz. These peaks can be regarded as index peaks for qualitative identification. By comparing the retention time and the on-line UV spectra of the samples with the chemical standards, three peaks were identified as protocatechualdehyde, caffeic acid and ferulic acid. The contents of caffeic acid in the compound dandelion enema and Taraxacum mongolicum Hand.-Mazz. were determined to be 63.7ng-ml'1~ lSOug-ml"1 and 0.015~0.040%, respectively. Finally, HPLC coupled with mass spectroscopy (HPLC/MS), a method with high sensitivity and specialty, was applied to obtain the fingerprint chromatography of the compound dandelion enema. From the ionic fragments of MS and retention time of each peak, main components were identified to be caffeic acid, ferulic acid and protocatechualdehyde.