Studies On The Hepatoprotective Effect Of Gossipium Hirsutum Extract On The Acute Experimental Hepatitis And Thrombolysis Effect In Vitro

Objective: To investigate the hepatoprotective effect of Flos Gossypium herbaceum extracts (FGF-â… , FGF-â…¡) on the acute experimental hepatitis induced by Paracetamol (PAR), D-Galactasamine (D-CalN), Bacillus Calmette-Guerin and Lipopolysaccharide (BCG+LPS) were observed. The dissolve effects on thrombus and fibrin plate in vitro were examined. Methods: Experimental hepatitis animal model were established in mice by single intraperitoneal injection of PAR (180mg/kg), in Wistar rats by a single intraperitoneal injection of D-CalN (350mg/kg) and in mice by caudal vein injection of BCG+LPS. The Alanine aminotransferase (ALT), Aspartate transferase (AST) level in serum and Malondialdehyde (MDA) , Nitric oxide (NO) content, Super oxide dismutase (SOD), Glutathione peroxidese (GSH-PX) activities in liver homogenate, the coefficient of liver and spleen were assayed and dimension of hepatic damage in the histopathology. Results: â‘ In experimental hepatitis animal model induced by PAR, FGF-â…  (35 mg/kg , 70 mg/kg) and FGF-â…¡ (35mg/kg , 70 mg/kg) was found to significantly decrease the serum transaminase activities (P<0.05 ) . Meanwhile, FGF-â…  (70, 35 mg/kg) having no significant effects on SOD, GSH-PX(P>0.05). But can decreased the MDA content (P<0.05) . FGF-â…¡ also decreased MDA content (P<0.05 ), and prevented the reduction of SOD , GSH-PX activities in liver (P<0.01 and P<0.05) .â‘¡In experimental hepatitis animal model induced by D-CalN, FGF-â…  (35 mg/kg, 70 mg/kg) and FGF-â…¡ (35mg/kg, 70 mg/kg) was found to significantly decrease the serum transaminase activities (P<0.01) . FGF-â…  (35 mg/kg, 70 mg/kg)increased the SOD activities(P<0.01 XFGF-I (70 mg/kg) decreased MDA content (P<0.05) , but having no effect on GSH-PX activity. FGF-I (35 mg/kg) increased GSH-PX activity (P<0.01) , but having no effect on MDA content (P>0.05) . FGF-II (35 mg/kg , 70 mg/kg) increased the SOD activity(P<0.05), increased GSH-PX activity(P<0.05 and P<0.01) and decreased MDA content (P<0.05 and P<0.01) .(3)In immunological hepatitis animal model induced by BCG+LPS, FGF-II(35mg/kg, 70mg/kg) was found to significantly decrease the serum transaminase activities (P<0.05). Meanwhile, FGF-II also decreased MDA, NO content (P<0.05) and prevented the reduction of SOD, GSH-PX activities in liver homogenate (P<0.05) and FGF-II (35mg/kg) decreased coefficient of liver (P<0.01) . FGF-I (35mg/kg , 70mg/kg) was found to decrease the serum ALT (P<0.01) , but cannot decreased the serum AST (P>0.05) , FGF-I (70 mg/kg) also decreased MDA (P<0.05) , but other index of liver homogenate FGF-I (35mg/kg ,70mg/kg) was ineffective (P>0.05) . ?FGF-I and FGF-II having no dissolve effects on thrombus and fibrin plate in vitro. Conclusions: FGF-II showed significant protective action on experimental liver injury in mice. FGF-I has less effect on protecting liver than FGF-H, though FGF-I has a little. In vitro, FGF-I and FGF-II has no dissolve effects on thrombus.