Inhibitory Effects Of Dog Bile On Dimethylnitrosamine-induced Liver Fibrosis In Rats

Objective To investigate the inhibitory effects of dog bile on liver fibrosis induced by Dimethylnitrosamine (DMN) in rat.Methods The rats were divided in to five groups often rats each. Exept the control group, all were given intraperitoneal injection of 1% DMN for 4 weeks. The treatment groups were given bear bile and dog bile C high and low dosage) solution by gastric gavage simultaneously. The liver/body weight ratio, levels of serum AST, ALT, total protein(TP) and Cholestrol were determined.H-E staining was used to observe the pathologic changes. Direct red staining was used to divide liver fibrosis grades, and determine the area of collageneous fibers by colcor image analysis system EDI and α -SMA expression were detected by irnmunohistochemical staining.Results Compared with the model group, the liver/body weight ratio was significantly increased (P<0.001), the levels of serum AST and ALT were significantly lower , and the TP content increased in dog bile groups. Histopathoiogical observation showed that the Dog bile has better suppressive liver fibrosis effects. The areas of collageneous fibers were significantly lower(P<0.01). and the number of ED1 and α —SMA-positive cells were markedly reduced thanthe toxic and bear bile groups.Conclusion Dog bile has better suppressive effects on DMN induced liver fibrosis in rat...