Study On Of Insulin-loaded Sodium Alginate Nanoparticles Preparation And Pulmonary Administration

Injection is the main strategy for administration of peptide or protein because of its instability under the effect of pH and enzyme. So the studies on the approaches of non-injection administration have been interesting and significant. Drug delivery systems (DDS) on nanoparticles for oral administration have been broad studied as the interesting field, which can improve the drug absorption after oral administration, prolong run action, control drug release, change the mechanism of drug absorption and in vive distribution of the drug.In this study, the nanoparticales containing insulin and alginic acid sodium, were prepared by solvent diffusion method. The properties of the nanoparticles on shape, size and zeta potential were investigated. The nanoparticle formulation was optimized with the size of 416.4nm, zeta potential of -91.4 ± 2.0mv and incorporation efficiency of 89.72% ± 3.9%.The in vitro release of insulin in the nanoparticles was investigated. Release medium contained glycin (as stabilizing agent) and sodium dodecyl sulfate (as desorption agent). It was found that burst release of insulin in the nanoparticles was reduced after the solidification of nanoparticles surface and release time was prolonged. 59% of insulin in the nanoparticles was released after 24h in the PBS (pH7.4)。Pharmacodynamic study was carried out by aspiratory route way administration in the diabetesmellitus rats, which were formed by the injection of streptozotocin. It was indicated that the blood sugar concentration was obviously decreased after the administration of insulin nanoparticles, with same Tniax, longer action time (Obvious Pharmacodynamic was obtained after 24h of administration) compared with hypodermal injection of insulin solution. The relative bioavailability was 61.64% compared with hypodermal injection of insulin solution. The content of exotic insulin was determined by the evaluation of C-peptide level in rat serum, which indicated that in vivo smooth concentration of insulin and prolonged action time were obtained compared with hypodermal injection of insulin solution.Some work was carried out about the insulin inhalant in order to promote the study of the inhalant of insulin nanoparticles. It was found that the good fluidity of particles was obtained using the lactose (74-1 OOum) as the carrier of insulin, with repose angle the of 39.57°. Settlement ratio of the mixture was 77.8% in the simulative aspiratory route way and 18.1% in the simulative lung. When using mannitol as the carrier of insulin for the inhale administration, the repose angle of the mixture which was prepared by, is 41.32°. Settlement ratio of the mixture was 72.9% in the simulative aspiratory route way and 38.0% in the simulative lung, which suggested that settlement ratio of the mixture prepared by spray drying in the simulative lung was significantly increased.