Exploration On The Pathogenic Genes And Novel Findings Of Osteosarcoma With Gene Microarray

Objective To explore the pathogenesis-related genes in osteosarcoma. To investigate the importance of gene expression to the pathogenesis of osteosarcoma. Methods 3 well-known ATCC osteosarcoma cell lines and an osteoblastic cell line were collected . After the total RNA extraction , the finally synthesized biotinylated cRNAs were hybridized to Affymetrix~? GeneChip~? U133A arrays. After the design and the synthethesis of the primers, ten of the differentially expressed genes were then chosen at random to further confirm the array results using the SYBR~? Green real-time RT-PCR method in nine freshly collected osteosarcoma specimens during operation. The data analysis depended on the ABI Prism 7000 system. Results using an entrance limit of ≥2. 0, there were 58 up-regulated and 142 dowm-regulated genes identified by microarray which were commonly differentially expressed among all the three osteosarcoma cell lines in comparison with the osteoblastic cell line hfobl. 19. Many of the genes were first reported to be related to thepathogenesis of osteosarcoma. The array results were further confirmed by the real-time RT-PCR. Conclusion Many genes were involved in the pathogenesis of osteosarcoma. It is helpful to apply gene array to find the laws of the gene expression and to study the gene-gene interactional relationships in this tumor.