Research On Vascular Smooth Muscle Cell Proliferation-related Genes In Diabetic Macroangiopathy Rats With Hypertension

The vascular smooth muscle cells (VSMCs) were recognized as central factor of diabetic macroangiopathy, which was occurred commonly in the early period of diabetic mellitus. Therefore, the definition on pathological mechanism of VSMCs proliferation must possess important significance for prevention and therapy of vascular complication. In this study, the rat cDNA microarray chip containing 4096 full length genes was used to establish the gene profile of VSMCs in diabetic macroangiopathy firstly. Then, the molecular biological techniques were used to firm the expression change of screened proliferation-related genes of cultured VSMCs stimulated by high glucose. In further, the RNA interfering was applied to silence the CD74 and Irf1. The biological characteristics of VSMCs after interfering were detected detailedly to explore the effects of interfering for CD74 and/or Irfl on the VSMCs proliferation in high glucose. Based on this study, the data suggest that the overgrowth of VSMCs in diabetic macroangiopathy rats with hypertension is a result of multiple gene regulation. Irfl may be an important or key gene responsible for proliferation of VSMCs in diabetic macroanigopathy.