Study On The Role Of CC-chemokine Receptor 5 In Experimental Autoimmune Encephalomyelitis

We aim to study on the role of CCR5 in experimental autoimmune encephalomyelitis. At first, we established EAE model in C57BL/6 mice by induced them with MOG35-55, and then the histopathologic changes were observed by using H&E and LFB staining. Our results showed that mice in the experiment group appeared CNS symptom in succession since 11 days post -immunization, and reached fastigium in 12 days after onset. The incidence was 88 percent. We found there were multiple inflammatory cells in the brain and spinal cords in EAE mice, and the demyelination changes in the white matter were evident. Subsequently, we observed CCR5 expression in spleen cells and spinal cords by FCM and IHC. We detected that CCR5 expression lever increased significantly in spleen cells, but there was no evident difference between EAE group and control group in the spinal cords. This study indicated that CCR5 may play a role in the early course of EAE.