Quantitative Analysis, Preclinical Pharmacokinetics And Toxicikinetics Of MCC-555 In Biological Matrix | Posted on:2007-10-03 | Degree:Master | Type:Thesis | Country:China | Candidate:N Sun | Full Text:PDF | GTID:2144360182491658 | Subject:Drug Analysis | Abstract/Summary: | PDF Full Text Request | A high performance liquid chromatographic method followed protein precipitation and liquid-liquid extraction respectively was developed and validated for the determination of MCC-555, a novel insulin sensitizer, in biological matrix. The calibration curves were linear in each sample range and both inter-day and intra-day RSD were below 15%. The method has been successfully used to support the pharmacokinetics and toxicokinetics study of MCC-555 in beagle dogs and rats. The absorption, distribution, metabolism and excretion of MCC-555 in animal were studied as well as in vitro binding of MCC-555 to human plasma proteins. The possible metabolites and path of MCC-555 metabolism in vivo and in vitro were supposed. And pathological histology observation of MCC-555 was also analyzed.
| Keywords/Search Tags: | MCC-555, high performance liquid chromatography, preclinical pharmacokinetics, toxicokinetics, tissue distribution, absorption, excretion, plasma protein binding, hepatic microsome enzyme, metabolite, pathological histology | PDF Full Text Request | Related items |
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