Expression Of HPOT1 Is Associated With Tumor Grade And Telomere Length In Glioma

PURPOSEWe have used realtime RT-PCR technique to compare differentially expressed hPOT1 genes among different grades' glioma and normal brain tissue in human and explored the relativity between hPOTl expression and telomere length. It might be helpful to understand the molecular mechanism of glioma formation, the regulated mechanism of telomere length. We want to know if hPOTl can be an index to determine glioma biology and prognosis. Our research may be helpful to gene treatment of glioma by molecular biology methods. And it may be a theorical basis to clinical engagement of more sensitive and more differential molecular tumor symbols.MATERIALS AND METHODSWe collected 53 glioma specimens excised in the surgery and 8 normal braintissues. All specimens were confirmed by pathological diagnosis. We measured hPOTl expression by realtime RT-PCR technique, at that time ACTB gene expression was an endogenous control. We measured telomere lengths of glioma specimens by means of Southern blot. RESULTSWe made data logarithmic transformation and then did homogeneity of variance estimation and LSD(Least-Significant Difference) estimation. We found hPOTl mRNA expression in the glioma tissue is obviously higher than it in the normal brain tissue.hPOT1 mRNA expression in the III/IV grade glioma tissue is higher than it inthe I/II grade. There is no obvious significance (P<0.05) . There is no obvious correlation between III/IV grade and I/II grade. POT1 expression levels showed raised in accordance with telomere shortening (r=-0.963,P<0.05) . CONCLUSIONThese data suggest that Potl may play an important role in regulation of telomere length, telomere maintaining and inhibition of Potl may induce telomere dysfunction. Moreover, changes in POT1 expression levels may be associated with glioma carcinogenesis and glioma progression. hPOTl mRNA expression level is significantly higher in glioma tumor than in normal brain tissue. Its raised level is different in different tumor tissues. We think it may be a sensitentive tumor marker. We consider that POT1 expression level has significant correlation with telomere length. It, in a certain extent, prooves the mechanism of POTL it is the basis to further study the function of POT 1 in the tumor formation and development.