The Study Of The Therapeutic Effect Of Pentapeptide Compound CMS010.26 On Rat Glomerulonephritis And Its Therapeutic Mechanisms

Objective:Investigate the therapeutic effects of pentapeptide compound CMSO 10.26 and its therapeutic mechanisms.Methods:1. Anti-GBM nephritis was established by injecting rabbit anti-rat GBM serum into rats.Rat mesangial proliferative nephritis model was induced by intravenous injection of anti-Thy1.1 IgG (0X7).2. All the animals were detected 24 hours urinary protein, serum BUN, CR. Kidney pathological damage was studied(including HE , PAS staining , immunofluorescence staining and electron microscopy examination).3. Culture rats mesangial cell HBZY-1. Study CMS010.26 effect on the mesangial cell proliferation and apoptosis ,PCNA expressed by mesangial cell was also studied.Results:1. CMS010.26 was used to therapy anti-GBM nephritis rats.The urinary protein decreased significantly from the 2nd to the 4th week. Serum BUN obviously lower than saline group from the 2nd week. Serum CR decreased significantly from the third week. All of the above results showed statistic significant difference, P<0.05. HE and PAS staining indicated that the level of glomerular crescents , glomerular swelling,cells in glomerular were all mild than saline group;Rat IgG, rabbit IgG deposited along with GBM were all less than saline group revealed by immunofluorescence staining. Electron microscopy examination indicated that the number of mesangial cells and ECM deposition were all obviously ameliorated than saline group.2. CMS010.26 was used to therapy rats MsPGN. The urine protein level decreased significantly from the 1st to the 4th week. Serum BUN and CR obviously lower than saline group from the 2nd week. All of the above results showed statistic significant difference, P<0.05. HE and PAS staining indicated that the level ofmesangial cell proliferation and mesangial matrix depostion were mild than saline group;Mouse IgG deposited in the mesangium region were all less than saline group revealed by immunofluorescence staining. Electron microscopy examination indicated that the number of mesangial cells and ECM deposition were all obviously ameliorated than saline group.3. CMSO 10.26 can inhibit the glomerular mesangial cell HBZY-1 proliferation and PCNA expression.CMSO 10.26 can induce HBZY-1 apoptosis.Conclusions:The experiment data showed that CMSO 10.26 could relieve nephritis kidney pathological lesion and urine protein level. By means of inhibiting mesangial cell proliferation and mesangial matrix production, inhibiting IgG deposition, inhibiting the mesangial cells proliferation and PCNA expression, inducing mesangial cell apoptosis,CMS010.26 ameliorated glomerulonephritis clinical and pathological damage.