The Biological Significance And The Related Molecular Mechanism Of Ets1 MRNA Expression In Lung Cancer TMA

Objective:To develop cell microarray and apply a rapid, normative, simple and economical method for the scientific research in cell fields and clinical work.Materials and Methods:We designed an 7X16 cell microarray, modulated the chest liquid to the proper consistence, handling the home-made cell microarray survey setting ( the patent appling No. 200520027814 0), put the liquid to the corresponding place with a 20 μl sample-shift , and then the cell microarray was done. We did HE stain, IHC, FISH after fixation and contrast the result to that from all the corresponding spread pieces. As a research method to the developing lung cancer, we take the statistic analysis between it to the tissue microarray with the x~2 test, and significance test value is 0. 05.Results:1 Result of HE stainThe made-up cell microarray is 112 points, 7X16, all points ranks in a good order without distort and distortion. The diagnosis resulted from the cell microarray was totally same to that from the corresponding spread pieces under the microscope. we found lung cancer cells in all the 50 cases.2 Result of FISH stainAfter FISH stain of Etsl mRNA, the number of cells did not decrease in evidence. The positive expression was totally same to that from the routine spread pieces.Comparing the positive expression rate of Etsl mRNA in the III + IV stages of lung cancer by the cell microarray to that by the tissue raicroarray, which is proof to be a valid method, we did not find the significant difference between them (P>0.05) .3 Result of IHC stainAfter IHC stain of c-Met, the number of cells did not decrease in evidence. The positive expression was totally same to that from the routine spread pieces.Comparing the positive expression rate of c-Met in the Hl+IVstages of lung cancer by the cell microarray to that by the tissue microarray, which is proof to be a valid method, we did not find the significant difference between themConclusions:1 The cell microarray can replace the routine cell spread pieces in the clinic diagnoses and scientific research, especially in the condition of a great many cases need to deal with.2 The cell microarray we made could be used in the research about chest liquid, ascites, urine, liquid in the heart fold, brain-ridge liquid, culture cells , et al. It is rapid, high specificity, high throughput, so it would be broadly used in the clinical diagnosis and the screen of epidemilology .3 The process is very simple and saving time. The microarray can be made soon after the case coming. And we have come true to handle the cases in different block to save the materials, keep the experiment condition standardization and reduce the system error.4 We can take the cultured cells to the cell microarray , it would play a great role to research the difference of the gene and protein in the different kinds of cells, to test the sensitivity of the medicine, to explore the toxicity of the drug and filtrate the gene clone in the gene transinfection.5 The cell microarray has its disadvantages, such as, it can not providethe formation of histology structure. Combined to the tissue microarray, it will play more important role in the scientific research.