Changes Of NF-κB In Rabbit Cardiomyocytes And LPO, SOD In Blood After Cardiopulmonary Resuscitation

ObjectiveNowadays the studys of reactive oxygen species and nuclear factor— kappaB are popular, there is more and more information to support reactive oxygen,especcial the superoxide , liquid peroxide and nuclear fators play a key role in ischemia — reperfusion injury. But the detailed channel that nuclear fators induce apoptosis and release reactive oxygen species still need the further study. Therefore, to investigate the alternative regulations of contents of LPO and SOD in blood and the content of NF—κB in myocardium before and after cardiopulmonary resuscitation(CPR) will have an important research and application prospects in to resist the ischemia— reperfusion injury after cardiopulmonary resuscitation.Subjects and Methods.Thirty healthy adult rabbits were randomly divided into three groups with 10 rabbits in each group:. The healthy rabbits were group C, the rabbits who didn't regain heart beats within 15 min were group F, and those who regain heart beats were group S. The model of the cardiopulmonary resuscitation in rabbit was established. The food and water was forbidden for rabbits before 18 hours of the operation. Under general anesthesia with E — barbital Sodium by the ear vein, rabbits were fixed on the board. To place the five—electrodes on the areas of the heart and observe ECG monitor series to record datas. Cut the skin of the front of neckthen exposed the trachea and the A. carotis communis. Separated the A. carotis communis and place a catheter washing with raloxifene (500U kg"1)* connecting with trauma — monitoring of blood pressure and pressure converters series to mearsure the mean arterial pressure (MAP). Separated and cut the trachea placing a trachealtube (4F children) , keeping indoor autonomous breathing. A catheter was inserted via the right femoral vein throught it intravenous. After a stable blood pressure had been obtained,we recorded the 5 min heart beat, breath rate,MAP and ECG. The tracheal tube was clamped at the end of breath to induce a model of cardiac arrest and loosed it in the 8th min. Ventilator assist breath, parameters of respiratory mechanics: at the rate of 20 compressions min"1, Tidal volumes were chosen with 15 to 20 mL kg"1' and the rabbits were injected with intravenous adrenaline at a dose of 15jug kg"1. Chest compressions were performed by the same investigator in all animals at the rate of 80 — 100 compressions min1. Observation end until spontaneous circulation returned or the 15th min of the start of CPR. lml blood samples were obtained in every group. Blood samples of group F were obtained after 15 min of cardiac arrest and quickly open the chest obtained myocardium organs for measuring the NF — kB;blood samples of group S were obtained after 15 min of spontaneous circulation returned and quickly open the chest obtained myocardium organs for measuring the NF—kB;blood samples of group C were obtained before the experiment began then opened the chest to obtain myocardium organs for measuring the NF—kB. Expressions of NF—kB were examined with s — p immuno-histo—chemistry methods. The activity of SOD was examined with xan-thine methods. Expressions of LPO were examined with TBA fluorescin methods. Data are expressed as mean (SD). All analyses were conducted using SPSS 10. 0 for Windows software . Datas were Analysed by repeated— measures analysis of variance (ANOVA) and t — test A P — value of less than 0. 05 was considered statistically significant.Results1. Contents of NF — kB compared with the control group: Contents of NF—kB of the group who didn't regain spontaneous circulation and the group who regain spontaneous circulation successfully both increased (Both P value<0. 01).2. Contents of LPO and SOD compared with the control group: Contents of LPO of the group who didn't regain spontaneous circulation and the group who regain spontaneous circulation successfully both increased (Both P value0. 05).4. Effects of NF — icB revealed with immunohisto — chemistry methods: There were few expressions of NF — kB in control groups measured with opuical microscopy;In the group who didn't regain spontaneous circulation, we can observed many particles and brown stripy depositions and a moderate expression;In the group who regained spontaneous circulation, we can observed more particles and brown stripy depositions and a high expression. Images were rendered to scan and calculation the mean optical density of each group. There were significant diferences in three groups.ConclusionsNF~K;B^LPO^SOD probably participate in pathophysiological courseof ischemia—reperfusion injury induced by CPR. It will be possible to reduce ischemia—reperfusion injury . The associated detections of the activation of SOD and the content of LPO and the level of NF— kB are helpful to find out the organism's immune state,and have guiding significant and value on inquiring into CPR's direction, prognosis and clinical treatment.