| Objective: To study the gene differential expression of taxol-resistant ovarian carcinoma cell line OC3/Tax300 compared with its parent cell OC3 and discuss the relationship between gene differential expression and Drug-resistance,Method: The cDNA retro-transcribed from equal quantity of mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively. The mixed probe were hybridized with BiostarH140S gene expression chip. The acquired image was analyzed by software.Results: 134 significantly differently expressed genes were screened out, of which up-and down regulated genes were 17 and 117 respectively. They were related to 14 kinds of genes. The down-regulated genes were EBNA-3,COP9, StIP1. The up-regulated genes were JAK2,HSPS,NADH, and so on.Conclusion: these differential expressed genes related to the mechanisms of taxol -resistance of ovarian cancer. |
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