Expression And Significance Of Heparanase MRNA In Tumoral Tissues And Exfoliated Urinary Cells Of Patients With TCCB

Objective: To study the expression and significance of heparanase mRNA in tumoral tissues and exfoliated urinary cells of patients with transitional cell carcinoma of bladder(TCCB) .Methods: Heparanase mRNA in tumoral tissues of 55 patients with TCCB, 28 normal bladder tissues, exfoliated urinary cells of 47 patients with TCCB, 5 patients with urinary cancers except bladder, 10 nonneoplastic urinary diseases patients and 10 healthy volunteers were detected. Heparanase and p-actin were amplified by using RT-PCR. Results: 1. The positive rates of Heparanase mRNA in TCCB and normal bladder tissues are 76.4% and 21.4% respectively. The expression level of Heparanase mRNA is elevated as the grade and stage increased in TCCB. The positive rates in T₁ , T_2-4 were 57.1%, 88.2%,in grade of G₁, G₂, G₃ were 47%, 81.5% , 100%,respectively(P<0.05);The positive rate of patients with carcinoma diameter more than 2cm in diameter was 90.0%, while was 60.0% in patients with carcinoma diameter less than 2cm in diameter, there was significant in relate with diameter (P<0.05). The expression difference of Heparanase mRNA was not significant in the tumor of other different clinical features (P>0.05). 2. 35 of 47 (74.5%) showed positive Heparanase mRNA signal in urine in patients with TCCB. Only 2 of 25 (8%) patients in control group showed positive Heparanase mRNA signals in urine. The expression of Heparanase mRNA difference between experimental group and control group was significant (P <0.05). The sensitivity and the specificity of the expression of HPSE mRNA in exfoliated urinary cells were 74.5% and 92%, respectively. There was not significant correlated with stage , grade and another clinical feathers . 3. Detection of Heparanase mRNA expression in tissue and urine in 30 patients with TCCB at the same time, The positive rates of Heparanase mRNA in tumor tissue and urine are 80.0% and 73.3% ,respectively (P >0.05). Conclusions: 1. Carcinoma tissues of the patients with TCCB showed significantly positive expression of Heparanase mRNA. Heparanase mRNA is correlated with stage, grade and size of TCCB. The up-regulated expression of Heparanase mRNA in TCCB suggests that Heparanase mRNA is tight related with the tumor occurrence and progressive. Therefore, we concluded that Heparanase mRNA might be the index for identifying grade, stage and recurrence of TCCB. Heparanase mRNA can provide new appropriate target for gene therapy to TCCB. 2. High sensitivity and the specificity of expression of urine Heparanase mRNA to provide a simple, noninvasive diagnostic test to identify patients with TCCB. There was not significant correlated with stage , grade and another clinical feathers. 3. There is no difference between the Heparanase mRNA expression of tumor tissue and urine.