| Objective: To investigate the effection and the mechanism of Endomorphine-1 on K562 in vitro.Methods: Inhibition of K562 cells proliferation was measured by MTT assay. Morphological assessment of apoptosis with Wright- Gimsa staining and fluorescence microscope. The apoptosis peak was measured by flow cytometry. DNA fragmentation was visualized by agarose gel electrophoresis. The expression of bcl-2 and bax was studied by immunohistochemistry through EM-1 on K562 cells.Result: Endomorphine-1 could time-dependently and dose-dependently inhabit the proliferation of K562 cells at concentration of 10-710-11 mol·L-1 for 96 h. Among them, the function of 10-8mol·L-1 is obvious, the cells treated with EM-1 showed characteristic of apoptosis under light microscope and fluorescence microscope. After a 96h incubation with EM-1 at the concentration of 10-8 mol-L-1, the percentage of apoptotic cells were 23.90±1.5 % . DNA agarose gel electrophoresis showed nuclear fragmentation (DNA ladder). The expression of bcl-2 was decreased in EM-1 treated cells , but the expression of bax increased.Conclusion: 1 Endomorphine-1 could induce K562 cells apoptosis, with time-dependently and dose-dependently.2 EM-1 could induce the apoptosis of K562 cells.3 The mechanism of action could be related to Bcl-2/Bax accessactivated. |
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