Role Of Thrombin Receptor In The Pathogenesis Of Spinal Cord Injuries In Rats

Objective : The study was to investigate the expression of thrombin receptor at different time after spinal cord injuring by heavy falling in rats. In order to explain that the thrombin if lead to the more injuries after spinal cord injuring. Discuss the depressor of thrombin was an availability treatment way. A research base is established for the clinical study.METHODS :Seventy-five adult male Wistar rats were randomly divided into 3 groups: (1) NS control group(NS group n=25):open the canalis vertebralis,but not injure spinal cord,and then inject NS 20ul. (2)Spinal cord injury group(SCI group n=25):open the canalis vertebralis,make spinal cord injury by 10g heavy falling from 8cm high. (3)Spinal cord injury and whole blood group(SCI-B group n=25): open the canalis vertebralis,make spinal cord injury by 10g heavy falling from 8cm high,at the same time,inject whole blood itself 20ul. Take out the spinal cord tissue from the rats, after making mold in different time(6h,24h,48h,72h,5d) in 3 groups, respectively, detected the PAR-1 expression(Immunohistochemical method). Obsreved the morphologic changes of the spinal cord tissue by H-E dye. Obsreved the morphologic changes of the neuron by transmissional electron microscopy. Detected the number of positive PAR-1 immunostaining cells in the spinal cord tissue by microscopy.Result :(1)There were few positive PAR-1 immunostaining neurons in NS group. (2)There was some positive PAR-1 immunostaining neurons in SCI group and SCI-B group. (3)The number of positive PAR-1 immunostaining neurons were markedly increased at 6h,peaked at 72h and decreased at 5d in SCI group and SCI-B group. (4)The neuron and tissue water contents wasevidence in SCI group and SCI-B group. ?There were apoptosis neurons at 72h in SCI group and SCI-B group.Conclusions; ?There were expression of PAR-1 at neurons of the spinal cord injury. (2) There was a rule of the expression: the number of positive PAR-1 of neurons were markedly increased at 6h,peaked at 72h and decreased at 5d. ?The nervous tissue was injured after the PAR-1 was activated,included: cell toxicity, tissue edema, apopotosis neuron.etc. ?The depressor of thrombin could decrease the tissue injury.