Expression Of Heme Oxygenase-1 And Heat Shock Protein (HSP) 70 In Renal Allograft Acute Rejection In Rats

Objective To investigate the expression of heme oxygenase-1 and heat shock protein (HSP) 70 in renal allograft of acute rejection in rats, to provide a theoretical base in the diagnosis and treatment of acute renal rejection, to help us explore new immunodepressives and induce immune tolerance.Methods We established the model of allogenic renal transplantation from SD rats to Wistar rats. End-to-end anastomosis of recipient's renal vein to donor's inferior vena cava was performed, End-to-side anastomosis of aorta was performed between donor's abdominal aorta and recipient's abdominal aorta, Urinary tract reconstruction was accomplished by suturing the donor's ureter with a bladder patch to the recipient's bladder. In four groups including normal control group, homogenic transplantation group (SD→SD) , allogenic transplantation group (Wistar→SD) and CsA group.The expression of HO-1 and HSP70 in kidneys was detected by immunohistochemistry, The correlation between HO-1,HSP70 and pathological findings was observed.Results The success rate of operation was 83 %.The vascular anastomosis time was 22±2 minutes, cold ischemia time was less than 65 minutes, warm ischemia time was less than 3 seconds. Total operative time was 150±10 minutes. The expression level of HO-1 and HSP70 in the homogenic transplantation group did not change greatly (p>0.05) .The level of HO-1 and HSP70 in the allogenic transplantation group was increased gradually on dayl,day3 and day7 (p < 0.01) . There was a significant correlation between the two factors and pathological index in the allogenic transplantation group (r=0.911,p<0.01,r=0.869, p<0.01) .Conclusion This model of renal transplantation is simple and reliable, and can be applied in the experiment of renal transplantation research. HO-1 and HSP70 may play an important role in acute renal allograft rejection and immunologic response of transplantation. Detection of HO-1 and HSP70 expression in allograft may be useful for early diagnosis and treatment of acute rejection.