| Objective: To clone the PDX-1 gene by genetic engineering, and gain the PDX-1 protein by amplification and establish the expression system. To establish the Wistar rat models of type 1 diabetes with streptozotocin, and inject the PDX-1 protein into the vivo of the rats, and then view the results. Method: First, we extracted the RNA and established the gene-library of the pancreas cells of the Wistar rat, and obtained the objective gene, pancreatic and duodenal homeobox gene 1 (PDX-1), recombinated the gene and the carrier, constructed gene expression system, induced gene expression ,then we separated and purified the PDX-1 protein. Second, we separate the Wistar rats of type 1 diabetes that were induced by streptozotocin into the experimental group and control group randomly, and injected the PDX-1 protein to the experimental group , injected equal amount of sodium chloride to control group,detected the fasting blood glucose of all the rats weekly, and detected the insulin, C peptide and the islet cells from the pathological section at the third month. Finally, we analyzed the data of the fasting blood glucose, insulin, C peptide and the islet cells of the experimental group and control group by the t-test with PEMS 3.1 statistical software. Results: It was confirmed by sodium dodecylsulphate and polyacryla-mide gel electrophoresis (SDS-PAGE), and staining with Coomassie brilliant blue, that the molecular mass of the PDX-1 coding protein iscoincident with the theory value: 30830.77. The fasting blood glucose level of the experimental group is lower than the control group from the forth week to twelfth week (P<0.05), and insulin and C peptide are higher than those of the control group at the twelfth week(P<0.05). The islet cells is more than it of the control group in the twelfth week. Conclusion: PDX-1 Protein can restore the pancreatic islet's function, regulate insulin secretion and blood glucose. It is a new direction for diabetic therapy. |
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