The Study On Urinalysis By Reagentless Spectroscopy

As a normal tool in clinical analysis, urinalysis has been widely used in hospitalbecause urine is easy to be collected without pain. The current chemical urinalysismethod is time consuming, contaminative and destructive and reagentless method inorder to facilitate the clinical application of urinalysis.In this thesis, middle infrared attenuated total reflection (MIR-ATR) spectrum, nearinfrared (NIR) transmission spectrum and near infrared flourier transform Raman(NIR-FT-Raman) spectrum were introduced into urinalysis. Multivariate statistics wasused to analyze spectra quantitatively.MIR spectrum is based on substance fundamental frequency oscillation, so SNR(signal to noise ratio) is higher than other two methods. The results from glucosewater solution indicated that RMSEP (root mean square error of prediction), anindicater for precision, was 13.69mg/dl, and correlation coefficient (R) was ulmostone. At the same time, the other components of urine disturb the predictive precisionin real urine model.NIR spectrum has more flexible measure conditions and much more information. Thetwo optical path lengths 0.7mm and 1.5 mm was used to model at 4200~4800cm-1and 5500~6500cm-1 respectively. The results were in accodance with the theory ofoptimum optical path length. In order to facilitate real time application in clinic, bythe use of optimum wavelengths, two sets of wavelengths, ten and six wavelengths,were used to improve the modeling.NIR-FT-Raman spectra of sigle components in urine are easily obtained. However,1064nm laser used in instrument made scattering light influence the region of NIRwater absorption. That induced the nonlinearity in spectra, therefore NIR-FT-Ramanis not suitable for the analysis of component in water solution.The results presented didn't reach clinical availability, and the methods introduced canbe used to pathologic urine screening. The further study should be done in order tooptimize model and thus improve the clinical availability of reagentless spectroscopyin urinalysis.