| Background: Insulin-like growth factor I contains of 70 amino-acids. IGF-II is consist of 67 amino-acids. Both of them are important hormones. They are associated with many activites such as the growth of tissue and cell, the metabolism of the body. 95 percent of IGF-I and IGF-II within body is combined with IGFBP-3, and the biological activity of which is modified mainly by IGFBP-3. IGFBP-3 is made up of 264 amino-acids and combines with IGF-I IGF-II and acid-labil sabunit to form complex which is comparatively stable and the molecular quality of which is 150×10~3. The function of the complex is to maintain IGF-I and IGF-II in certain level in the serum and prolong their t1/2. IGF-I, IGF-II and IGFBP-3 are mainly producedby liver cells, Therefore the status of liver function determines the serum level of IGF-I, IGF-II and IGFBP-3. Is there any difference between the level of IGF-I, IGF-II and IGFBP-3 in patients with posthepatitic cirrhosis and alcoholic cirrhosis? Though there are some authors who did some work on this subject but not give clear answer. We detected the serum level of IGF-I, and IGFBF-3 with radioimmunoassays and IGF-II with enzyme-linked immunosobent assay in order to observe whether there is some differeence between these two groups.Materials and methods: All of the cases were from Jinhua TCM Hospital and Jinhua Center Hospital during 2003,5-2005,9. There were 45 cases in the group of alcoholic cirrhosis, 60 cases of posthepatitic cirrhosis and 30 healthy subjects served as controls. All the cases were male. 6ml vein blood was obtained from each case, after a 12th fast, of which the serum was separated and kept at -30 °C in refrigerator escaping from being frozen and melt again. The treatment of the Samples was strictly according to the instructions of boxes. The concentrations of serum IGF-I and IGFBP-3 were measured with radioimmunoassays. The boxes were from Biocode. The concentration of IGF-II was measured with enzyme-linked immunosobent assay. The boxes were from Rapidbio.Results: The levels of IGF-I IGF-II and IGFBP-3 in the group ofposthepatitic cirrhosis were 69.42 ± 50.13ng/ml, 13.41 ± 9.55ng/ml and 1321.28+920.15ng/ng/ml, and that in the group of alcohol cirrhosis were 50.45 ± 42.87ng/ml 8.51 ± 6.24ng/ml and 1025.09 ± 808.27ng/ml, in the controls 201.91 ±72.48ng/ml. 24.25 ±10.39ng/ml and 2125.92 ±762.25ng/ml. The level of the previous three subjects in the group of alcoholic cirrhosis were statistically higher than that in the group of posthepatitic cirrhosis ( p<0.05 ) . The levels of the subjects in controls were higher than that in two cirrhosis groups (pO.01) . The three subjects dropped gradually according to disease severity (child pugh score) from group A to B and C in cirrhosis groups (p<0.05 ). The levels of each of the three subjects in the classification of child pugh An B and C in the group of alcoholic cirrhosis was respectively higher than that in the patients with posthepatitic cirrhosis. ( p<0.05 )Discussion: The levels of IGF-I and IGFBP-3 dropped obviously in the case of cirrhosis because of the severe resistance to GH in the body which causing the failure of the modifying of the GH/IGF-I. The hepatocellular disfunction of the patients with cirrhosis and the bad nutritional status caused by portal flowing which made the liver cell to produce little IGF-I and IGFBP-3. The decrease of the forming of IGF-II in liver was associated closely with hypohepatia in the status of cirrhosis since the gene of IGF-II was expresed specially in the tissue of liver.It was shown in this paper that the levels of IGF-I IGF-II and IGFBP-3 were associated with the classification of child pugh. The levels of the three subjects dropped gradually according to the status of liver function which suggestted that the quantity of IGF-I IGF-II and IGFBP-3 produced and secreted by liver dropped corresponding to the severity of cirrhosis. This suggestted that the previous three subjects were useful indicators to evaluate the status of liver function.It was illustrated on this paper that the levels of IGF-L IGF-II and IGFBP-3 dropped more slowly in alcoholic cirrhosis than in posthepatitic cirrhosis, combining with the history of the case and other characters, which might help to identify alcoholic cirrhosis from posthepatitic cirrhosis and the reason is waiting for further research.Conclusion: The levels of serum IGF-I IGF-II and IGFBP-3 in alcoholic cirrhosis and posthepatitic cirrhosis dropped obviously and the decrease at later was obvious comparatively. The levels of the previous three subjects dropped gradually from child pugh Ato B and C in cirrhosis according to the disease severity. IGF-I IGF-II and IGFBP-3 were useful indicatoes to evaluate the severity of cirrhosis and might help to identify alcoholic cirrhosis from posthepatitic cirrhosis.
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