Isolation Of Prostate Cancer Metastatic Cells And Screening Of The Metastasis-associated Genes

[Objective]To isolate high metastatic and low metastatic prostate cancer cells and screen metastasis-associated genes for the purpose of the biomarker exploration of prostate cancer metastasis.[Methods]1. Fresh prostate cancer specimens and DU145 human prostate cell line were cultured in vitro, respectively. Morphological and karyotypic characteristics, doubling time and anchorage-independent growth were then evaluated.2. Fresh clinical specimens / cancer cell suspensions were transplanted orthotopically, s.c, or under the renal capsule into BALB/c nude mice for isolation of the high metastatic and the low metastastic prostate cancer cells. Immunohistological analysis for the expression of putative metastasis-associated molecules were performed in the high metastatic and the low metastatic prostate cancer from the nude mice.3. Differential profiling of gene expression between the high metastastic and the low metastastic prostate cancer cells were screened by cDNA chip.[Results]1. Spontaneous metastastasis models of DU145 cells in nude mice were successfully established, and the high metastastic cells from lung, liver and lymph node were isolated and cultured.2. Immunohistochemistry analysis showed that expression of HER-2, AR were higher in the high metastastic prostate cancer tissues than that in the lowmetastastic tissues from the mouse models.3. Two hundreds and eighty four differential expressed genes between the high metastasitc and low metastatic prostate cancer cells were screened by cDNA chip. Those genes involved in signal transduction, cell cycle, apoptosis, transcription, adhesion, metabolism, etc.4. Efforts in establishment of cell lines and mouse model were not successful by using the fresh clinical prostate cancer samples.[Conclusion]High and low metastastic prostate cancer cells were obtained by screening in nude mice for several cycles. More than 200 differential expressed genes might be associated with metastasis of human prostate cancer. This study provided references for mechanism exploration of prostate cancer metastasis.