Establishment Of Aconite Processing Standard And Initial Research Of The Processing Technical

The difficult point of TCM modernization is the quality control of TCM. Having the feature of entirety, mark and stability, Finger print of TCM is a new controlling pattern of TCM. It can react the internal quality of medical material, and is accepted as the effective device by international.TCM origin from the natural world. Some of them are stimulus or toxic, some of them have different effect through different method of processing. So many of them should be processed before used in clinical.TCM processing has long history, many processing method is still based on traditional, empirical method. There still has no authority and unity standard about TCM processing. In this article, aconite, a toxic TCM, is the research target. How to applicate the finger print of TCM in the processing of aconite is focused. Based on these work, providing a investigate idea and scitific evidence.Aconite is a wildly used traditional Chinese medicine, it can expel wind-evil and remove wetness, warm meridian and alleviate pain. But the material is toxical seriously, only by processing can it be used in clinical because the toxical double-ester type aconitine change into single-ester type aconitine or aconite archi-ester. The dosage of processing acotine should increase when they are used in clinical and will display other pharmacological action, such as hadro-heart, boost pressure and so on. Thus, it is necessary for aconite to be processed.Currently, there are many literatures about how to determine the content of three main aconitine in aconite have been reported, but literatures on how to control the qulity of processing aconite are less. The general processing method of aconite is: Separating aconite according big or tiny, soaking them in water until no dry part inside. Taking out of them, boiling them in water for 4~6h or parboiling them for 6~8h until no white part in big and solid center ones. When tasting benumbing a little, taking out of them, airing them until half dry or so, slicing them and drying them. Obviously, "tasting benumbing a little" is not a objective, scientific standard.This article researches 5 toxical double-ester aconitine, Firstly, optimizing theextract condition by orthogonal design to determine the best extract method of alkaloid in aconite. Then setting up HPLC method of 5 double-ester aconitine. Analyzing the spallation of double-ester aconitine by means of ion trap MSn . explaining the rationality of processing. At last giving the peak belongs.On the research of aconite processing, the focus lies on how different soaking time and different solution influences the content of aconitine. At the end of this atical, setting up a object standard that equals to "tasting benumbing a little ".