Effect Of 6% Hydroxyethyl Starch On The Plasma Interleukin-6,Interleukin-10 In The Laparoscopic Surgery Patients

ObjectiveSuch as traumata and shock, operation can cause the restraint and disharmony to immune system with anaesthetic drug. They all have effects on the cy-tokine(CK).In this study, we use Enzyme Linked Immunosorbent Assay (ELISA ) technique to study the effects of Hydroxyethyl starch(6% HES,200/0.5)on the expression of interleukin - 6 and interleukin - 10 in the Laparoscopic Surgery patients.MethodsThirty - six ASA I - II female patients undergoing Laparoscopic Surgery were randomly allocated to two group, Hydroxyethyl starch group ( H group, n = 18) and Ringer's solution group(R group,n = 18). 10ml·kg^-1 HES or 15ml ·kg^-1 Ringer's was infused before surgery with rate 20ml·minute^-1 followed with Ringer's 10 ml·kg^-1 · h^-1. Before induction(T₁) ,at the begin (T₂)and the end of surgery(T₃) ,4 hours (T₄) and 24 hours(T₅) after Laparoscopic surgery ,plasma IL—6 and IL — 10 level were measured by Enzyme Linked Immunosorbent Assay ( ELISA).Statistic management: Paired design t test was used to compare in each group;two independent sample t - test was used to cmpare with group H - R. P < 0. 05 means significant differences.ResultsThree patients were abandoned o The two group were cmparable with age, body weight,duration of operation and the amount of 6% HES and Ringer's solution 0 In the two groups, plasma IL - 6 was significant elevation at the end of surgery and 4 hours after Laparoscopic surgery (P < 0. 05) , but no significant difference exists when comparing between two group0 Following infusion of 6% HES 10ml ? kg" ,there is still significant elevation of plasma IL - 10 level at T4 and T5 by comparing before induction and R group( P < 0. 05). IL - 6/IL - 10 ratio increased significant at the end of operation in R group (P <0.05).DiscussionsInterleukin - 6 is the representative cytokine of promote inflammatory mediators ,it can trauma economy immunity o Interleukin - 10 is the representative cytokine of resist inflammatory mediators.In this study,we tested the expression of IL -6 and IL - 10 in the Laparoscopic Surgery patients. We found that infusion of 6% HES can improve the expression of IL — 10 and keep the balance of promotable to contradict inflammatory mediator.ConclusionsInfusion of 6% Hydroxyethyl starch 10ml "kg""1 can improve inflammatory mediator - interleukin - 10 and also activation on the balance of immunity. 6% HES can maintain hemodynamics stable.