Treatment Of Coleitis And Antibacterial Capability Of Hypothermic Lysozyme From Marine

AIM To investigate the antibiotic capability of hypothermic lysozyme from marine in vitro and observe the treatment and mechanisms of it on the rat endocolpitis caused by bacterium. MATHODS By using minimal inhibitory concentration (MIC) and minimal bactericidal concentration methods, we tested the antibiotic capability of hypothermic lysozyme on F.sporutum CMCC( B )64941, C. albicans CMCC( F )98001, gram positive and gram negative bacteria in vitro. And also, we tested the curing rate of the lysozyme on endocolpitis of the rat according to negative rate of the vaginal secretions. We observed the mechnism of antibacterial effect of the lysozyme by transmission electron microscope. RESULTS The hypothermic lysozyme has antibiotic capability on anaerobe and fungion: F.sporutum CMCC( B )64941: 0.0156%. C. albicans CMCC ( F) 98001: 0.0625%. The hypothermic lysozyme also has antibiotic capability on G~+ G~-bacteria: The range of Staphylococcus aureus was 0.25- 16ug/ml, MIC50: 4ug/ml, MIC90: 16ug/ml;Staphylococcus epidermidis from 0.0625 to 8ug/ml, MIC50: lug/ml, MIC90: 4ug/ml;Enterococcus: 0.25 - 16ug/ml, MIC50: 4ug/ml, MIC90: 16ug/ml, E.coli: 0.125 - 32ug/ml, MIC50: 4ug/ml, MIC90: 32ug/ml;P.aeruginosa: 0.0625 - 32ug/ml, MIC50: 2ug/ml, MIC90: 8ug/ml;K.pneumoniae: 0.125 - 16ug/ml, MIC50: 2ug/ml, MIC90: 16ug/ml;Acinetobacter: 0.25 - 32ug/ml, MIC50: 4ug/ml, MIC90: 32ug/ml. The MBC of the Lysozyme on 8 strains of bacteria were as following: SA (JP - 04 - 08): 0.5mg/ml. SA ( ATOC25922): 0.25mg/ml;P.aeruginosa ( LN-04-12 ) : 0.25mg/ml;P.aeruginosa (ATOC27853 ) : 0.5mg/ml;E. coli (DC-04-21 ) and ( ATOC25923 ) : 0.25mg/ml and 0.5mg/ml respectively;Se (BP-04-18 ) :0.25mg/ml;P. vulgaris (BX-04-10) : lmg/ml. Three doses of lysozyme ( 10mg/kg, 5mg/kg,2.5mg/kg ) had treatment effects on endocolpitis of the rat caused by the combination of SA and E.coli. The cure rates were 92.9%, 82.1% and 64.3% with a dose-dependent manner. Normal E.coli showed straight or rod shapes. Their cell walls and membranes were integrated and smooth with tight constructions. The membranes stick to the walls and cytoplasm were uniform. However, after affected by lysozyme for 1h, the cell walls were lacked and had reductus with pyknosic cytoplasm. 2 hours later, mostcells became global shape and 3 hours later, the cell walls depleted and the cells spitted. It can refer that its action'place is at cell wall. CONCLUSION The hypothermic lysozyrhe from marine had obvious antibacterial effects on many strains of bacteria. Thus it has a wide antibacterial spectrum. Its effective location on the bacteria is their cell walls. Three doses of lysozyme range from 2.5mg/kg to lOmg/kg had dose dependent treatment effects on endocolpitis of the rat caused by the combination of SA and E.coli. The cure rates were 92.9%, 82.1% and 64.3%.