Expression Of Interferon-α Receptor And Proliferating Cell Nuclear Antigen In Craniopharyngioma

Objective To investigate the expression of interferon-a receptor (IFN-aR) and proliferating cell nuclear antigen (PCNA) in different pathological types of craniopharyngiomas, and explore their significance of the expression of IFN-aR and PCNA in craniopharyngiomas.Methods SABC immunohistochemical technology was employed to examine the expression of IFN-aR and PCNA in fifty-five cases craniopharyngiomas of different pathological types and ten cases of normal brain tissues. The differences of the expression of IFN-aR and PCNA in craniopharyngiomas of different pathological types were analyzed with SPSS 10.0 statistical software.Results Immuohistochemical expression rates of IFN-aR and PCNA were 69.09% and 76.36% in fifty-five cases of craniopharyngiomas(including 17 cases squamous papillary craniopharyngiomas, 27 cases adamantinomatous craniopharyngiomas and 11 cases recurrent adamantinomatous craniopharyngiomas) respectively, and were negative in ten normal brain tissues. The positive rate of IFN-αR was 81.58% in adamantinomatous craniopharyngiomas and 41.18% in squamous papillary craniopharyngiomas.The difference was statistically significant (x~2=8.9780, P<0.05);The positive rate of PCNA was86.84% in adamantinomatous craniopharyngiomas and 52.94% in squamous papillary craniopharyngiomas that did not give rise to postoperative recurrence in these samples.The difference was statistically significant (x~2 =7.4787, P<0.05). In adamantinomatouscraniopharyngiomas, expression of averagely positive cells of IFN-αR was (62.0±13.5)% in recurrent group and (53.2±18.6)% in non-recurrent group. The difference was not statistically significant (t=1.4192,.P>0.05);PCNA labeling indices (PCNA-LI) were (49.0±18.9) in recurrent group and (34.8±21.5) in non-recurrent group. The difference was not statistically significant (t=1.9076, P>0.05). The positive rate of IFN-αR was positively correlated with that of PCNA in craniopharyngiomas(r= 0.8318, P <0.01). Conclusions (1) Expression of IFN-αR is found in the majority of craniopharyngioma. The expression rate of IFN-αR was much higher in adamantinomatous craniopharyngiom-as than in squamous papillary craniopharyngiomas and in normal brain tissues, i. e. The expression of IFN-aR is present different in craniopharyngioma cells with different pathological features, which indicates that The increased expression of IFN-aR opens new possibility that IFN-a and IFN-aR can regulate cell proliferation of craniopharyngiomas, especially of adamantinomatous craniopharyngioma.(D The expression rate of PCNA was much higher in adamantinomatous craniopharyngiomas than in squamous papillary craniopharyngiomas and in normal brain tissues. Craniopharyngioma cells with different pathological features may possess varied proliferating potentials, and active proliferation and invasive potencials in adamanti- nomatous craniopharyngiomas are the main factor for postoperative recurrence of craniopharyngioma, which contributes to evaluating the prognosis of craniopharyngioma. (D The positive rate of IFN-aR is positively correlated with that of PCNA in craniopharyngioma, which suggests that IFN-R might be involved in the pathway of craniopharyngioma differentiation and be correlated with proliferation of craniopharyngioma cell.? Effective function of type I IFN depends on the integrity of cellular membrane receptor. Density of IFN-aR may be a considerable factor that IFN-a can adjuvantly treat craniopharyngioma.