The Effect Of Growth Hormone And Somatostatin On Breast Carcinoma Implanted In Nude Mice

Objective:To investigate the effect of Growth Hormone (GH) and Somatostatin (SST) on breast carcinoma implanted in nude mice.Methods:MCF-7 cells were implanted into the right axillary fossa's subcutaneous tissue of 40 nude mice. After the model of breast carcinoma was established, the animals were randomized into 4 groups:the GH group,SST group,GH+SST group and the control group, with 10 mice in each group. Tumor growth was observed. After these were administered, all mice were put to death. The weight of the transplantation tumor was observed. The structure of the tumor was observed with microscope and the expression level of Ki-67 and Bcl-2 which detect the proliferation and apoptosis of tumor cells were examined by immunohistochemistry. Then we compared the treated groups and the control group.Results: 1. The change of weight in nude miceThe weights of nude mice in 4 groups before and after administration were: NS group 19.22±1.41g,20.07±1.60g;GH group 19.73±1.22g,21.27±1.43 g;SST group 19.02±1.53g , 20.19±1.72 g ; GH+SST group 20.14±1.05g ,21.64±0.72 g. We made a statistics analysis to the difference of nude mice's weight among all groups, the P value was 0.048, We compared by twos further, and find when compared GH group with NS group P value was 0.014, when compared GH+SST group with NS group P value was 0.020 while the others P values larger than 0.05.2. The volume change of transplantation tumor in nude mice after administration...