The Mechanism Study On The Apoptosis Induced By TRAIL In The Leukemia Cell Lines K562 And U937

Objectives: Tumor Necrosis factor-related apoptosis-inducing ligand (TRAIL) is a new member of TNF super-family discovered recently. TRAIL can induce apoptosis preferentially in a variety of tumor cell lines, but can not affect the normal cells. Few studies have reported about the influence of TRAIL on tumor cells of haematological system. In this study, we discuss wheather TRAIL can induce apoptosis in leukemia cell lines. We apply TRAIL alone or together with DNR to induce the apoptosis of the leukemia cell lines K562 and U937 compared with the normal control cell line. The mRNA expression of four TRAIL receptors DR4, DR5, DcR1, DcR2 are deteced, controlled with normal cells through detecting the apoptosis effect of combined use of TRAIL and DNR or alone to explore the mechanisms which combined with chemotherapy could up-regulate sensitivity to therapy. TRAIL may be act as a new drug of biologic therapy and it has a promising prospect in clinical use of leukemia.Methods: 1 Study on the apoptosis treated by TRAIL alone in U937 cellline.